The hyaluronan receptor CD44 is an abundant glycoprotein expressed on a variety of different cell types. In fibroblasts a significant portion of receptor molecules remain in the detergent-insoluble fraction after Triton X-100 extraction. Detergent insolubility of these CD44 molecules has been interpreted to reflect their association with the cytoskeleton. In this study we examined the structura...

Triton X-100 treatment or freeze-thawing damages the membranes of sporulating or vegetative cells as seen by protein leakage from cells. A 40% increase in the specific [(14)C]benzylpenicillin-binding capacity of detergent-treated or frozen sporulating cells was observed. Neither freezing nor Triton X-100 treatment of vegetative cells produced a detectable effect on their [(14)C]benzylpenicillin...

objective: microbial plaque is among the main etiologies of periodontal disease, and tooth brushing with toothpaste is the most commonly used method of plaque control. this study aimed to compare the efficacy of tooth brushing with and without three different dentifrices.   methods: thirty dental students were randomly selected to participate in this clinical trial and were asked to refrain fro...

the effect of phosphorus supplementation to ammoniated rice straw was studied. the in vitro experiment was carried out following the first stage of tilley and terry method. the treatments consisting of four diets were a= 50% ammoniated rice straw + 50% concentrate (control), b= a + 0.2% phosphorus (p) supplement, c= a + 0.4% phosphorus (p) supplement, and d= a + 0.6% phosphorus (p) supplement o...

Purpose of the experiment was to evaluate effect lemon-seed essential oils on chemical composition and in vitro degradability alfalfa silage. Treatments were silage with no additive (control) or treated 60 ml/kg DM (C60), (C120) equal mixed them (M60). Whole plant ensiled for d triplicate laboratory scale tubes. Dry matter, organic crude protein, insoluble fiber acidic neutral detergent, water ...

The effects of different additives, including lactic acid bacteria inoculants, acremonium cellulase and mixtures of lactic acid bacteria inoculants and acremonium cellulase on changes of nutritive components and in vitro digestibility of Ceratoides arborescens during ensiling were investigated. The silages of each treatment were prepared in plastic film bags, kept in an incubator at 30°C and ba...

When cell membranes are treated with Triton X-100 or other detergents at 4 degrees C, a nonsolubilized fraction can often be recovered, the "detergent-resistant membranes", that is not found when detergent treatment takes place at 37 degrees C. Detergent-resistant membranes may be related in some cases to membrane "rafts". However, several basic aspects of the formation of detergent-resistant m...