This study describes the feasibility and adjuvant mechanism of a degradable emulsion for tuning adaptive immune responses to a vaccine antigen. We featured a mouse model with ovalbumin (OVA) as the antigen to deepen our understanding of the properties of a degradable emulsion-based adjuvant, dubbed PELC, interacting with immune cells and to elucidate their roles in vaccine immunogenicity in viv...

Extended life expectancy and medical development has led to an increased reliance on biomaterial implants and devices to support or restore human anatomy and function. However, the presence of an implanted biomaterial results in an increased susceptibility to infection. Due to the severity of the potential outcomes of biomaterial-associated infection, different strategies have been employed to ...

Two finishing trials and a metabolism trial were conducted to evaluate level of supplemental degradable intake (crude) protein (DIP) in finishing diets on cattle performance, carcass characteristics, and ruminal metabolism. Finishing trials were conducted in two consecutive years using 128 crossbred yearling steers (BW = 343+/-5 kg, Trial 1) and 176 crossbred yearling steers (BW = 375+/-4 kg, T...

The effective delivery of protein therapeutics remains a major challenge. Here, the novel nanoplatforms with bovine serum albumin (BSA)-coated, Dnase-1-loaded MnOx nanoflowers are developed (denominated MD@BSA). These MD@BSA present highly efficient loading and Dnase-I into tumor cells. Under glutathione (GSH) overexpression environment, is degradable, resulting in Dnase-1 Mn2+ release for comb...

Three ruminally cannulated and multicatheterised lactating dairy cows were used to investigate the effect of different supplement strategies to fresh clover grass on urea and short-chain fatty acid (SCFA) metabolism in a zero-grazing experiment with 24-h blood and ruminal samplings. Fresh clover grass was cut every morning and offered from 0800 to 1500 h. Maize silage was fed at 1530 h. The thr...

Soluble protein antigens used in vaccines have shown lower immune responses when compared with certain particulate forms of these same antigens. For example, it has been shown that microand nano-particle mediated delivery of protein antigen can use up to 100 times less protein and still produce an effective immune response [1]. In order to use this phenomenon to make vaccines more efficient, we...

Tissue engineering strategies for repairing and regenerating articular cartilage face critical challenges to recapitulate the dynamic and complex biochemical microenvironment of native tissues. One approach to mimic the biochemical complexity of articular cartilage is through the use of recombinant bacterial collagens as they provide a well-defined biological 'blank template' that can be modifi...