Background: In vitro maturation (IVM) of immature oocyte is a promising treatment option for infertile women.IVM procedures attempt to mimic the natural conditions of the follicular environment. The technique of IVM of oocytes has been successful after the first trial using culture of human oocytes in follicular fluid. The crucial part of this technique is the maturation process itself, and the...

introduction: standard concentrations of antibiotics in culture media are thought to have no detectable toxic effects on the cultured cells. however, since antibiotics are biologically active substances, the possibility that they interfere to some extent with cellular processes occurring in the cultured cells can not always be totally excluded. this study, therefore, was conducted to assess whe...

Research on early bovine embryonic development in vitro has been done. Oocytes were collected by aspiration technique from slaughter house ovarian and classified based on the number of cumulus cell layers and homogenous of cytoplasm. Maturation media consisted of tissue culture medium (TCM) 199 supplemented with 10 IU/ml pregnant mare’s serum gonadotropin (PMSG), 10 IU/ml human chorionic gonado...

SummaryAiming to improve in vitro production of bovine embryos and to obtain supplements to replace serum for in vitro maturation (IVM), this study evaluated the effects of macromolecular supplementation of IMV medium (bovine serum albumin - BSA, polyvinyl alcohol - PVA, polyvinyl pyrrolidone - PVP, Ficoll, KnockoutSR, or fetal calf serum - FCS) and oxygen tension [5% CO2 in air (20% O2) or 5% ...

In-vitro maturation can have deleterious effects on spindle formation and proper chromosome alignment in human oocytes and can be profoundly affected by culture conditions. This study compared the spindle presence and location with the maturation rate of germinal vesicle (GV) oocytes cultured in two different media: G1.2 and G1.2 supplemented with follicle-stimulating hormone, human chorionic g...

in vitro maturation (ivm) is emerging as a popular technology at the forefront of fertility treatment and preservation. however, standard in vitro culture conditions usually increase reactive oxygen species (ros), which have been implicated as one of the major causes for reduced embryonic developmental competence. it is well-known that higher than physiological levels of ros triggers granulosa ...

introduction: dehp [di(2-ethylhexyl) phthalate)] is widely used in plastic industry and some reproductive toxicity has been shown with it. so, this study was designed to evaluate dehp effects on resumption of meiosis and in vitro maturation of mouse oocytes as well as development of embryos resulted from them. material and methods: mice of 4-6 weeks old were administered daily doses of 50, 100,...

dendritic cells (dcs) induce pathogen-specific t cell responses. we comprehensively studied the effects of addition of maturation stimulus, fibroblasts (fibroblast conditioned medium), pha activated t cells (t cell conditioned medium), and mixture of fibroblast & pha activated t cells (fcm-tccm) conditioned media on maturation of dcs. monocytes were cultured with gm-csf and il-4 for five da...

Background: This study examined the effect of including one of two growth factors (100 ng/ml IGF-1 or 20 ng/ ml EGF) in combination with one of two antioxidants (50 μM cysteamine or 50 μM beta-mercaptoethanol) in invitro maturation of buffalo oocytes, followed by fertilization and subsequent development of embryos. Materials and Methods: The oocytes were recovered by in vivo ovum pick-up techni...