N-Acetyl-fi-D-glucosaminidase activities were determined in homogenates of marmoset kidney, in serum and in urine by using the 4-methylumbelliferyl substrate. The enzyme activity was separated into several components by DEAE-cellulose ion-exchange chromatography, starch-gel electrophoresis and isoelectric focusing. The kidney contained two major forms of the enzyme, A and B, which had similar p...

A fraction obtained from detergent-extract of sea urchin or starfish spermatozoa using DEAE-cellulose chromatography reactivated Triton X-100 models of the spermatozoa in a cAMP-dependent manner. The DEAE fraction contained cAMP-dependent protein kinase with a high level of specific activity. Rabbit muscle inhibitor protein highly specific for cAMP-dependent protein kinases inhibited the abilit...

Rabbit liver UDP-glucuronyltransferase activity was resolved into two separate fractions on DEAE-cellulose, one containing most of the transferase activity toward oestrone and the other most of the activity toward p-nitrophenol. These two activities were completely separated by rechromatography of each fraction on a second DEAE-cellulose column.

-In rats, the acute oral LDs0 value of neutralized 2-N-monoethylaminoethanol (MEAE) was found to be 1 "0 (0.68-1 35) g/kg. The acute oral toxicity of MEAE, but not that of 2-N-diethylammoethanol (DEAE) could be largely reversed by simultaneous or subsequent ingestion of choline. MEAE partially prevented the development of fatty hver in chohnedeficmnt rats. No reversal of depressed growth rate w...

The need for alternative and environmental friendly methods of waste clean-up has led to the use enzymes in bioremediation. In this study, white rot fungi were isolated from decaying plant parts using standard microbiology biochemical techniques. fungal mycelial identified screened substrates (2, 6-DMP) determine their capability production Manganese peroxidase. Pure manganese peroxidase was ac...

A procedure is described for obtaining DNA-dependent RNA polymerase form B from calf thymus in high purity. The procedure includes homogenization in low salt, sedimentation of chromatin at 50 000 x g, adsorption to DEAE-cellulose in a batch procedure, DNA-agarose chromato­ graphy, and chromatography on DEAE-Sephadex. Analyses of the preparation by band sedimenta­ tion and gel-electrophoresis in...