Escherichia coli cells normally require RNase E activity to propagate and form colonies. Using random Tn10 insertion mutagenesis, we screened for second-site suppressor mutations that restore colony-forming ability (CFA) to E. coli cells lacking RNase E function and found mutations in three separate chromosomal loci that had this phenotype. Restoration of CFA by mutations in two of the genes id...

In 1981 Claude Berge asked about combinatorial properties that might be used to solve Hex puzzles. In response, we establish properties of dead, or negligible, cells in Hex and the Shannon game. A cell is dead if the colour of any stone placed there is irrelevant to the theoretical outcome of the game. We show that dead cell recognition is NPcomplete for the Shannon game; we also introduce two ...

Arterial isocapnia is a hallmark of moderate exercise in humans and is maintained even when resting arterial Pco(2) (Pa(CO(2))) is raised or lowered from its normal level, e.g., with chronic acid-base changes or acute increases in respiratory dead space. When resting ventilation and/or Pa(CO(2)) are altered, maintenance of isocapnia requires active adjustments of the exercise ventilatory respon...

The recognition and clearance of dead cells is a process that must occur efficiently to prevent an autoimmune or inflammatory response. Recently, a process was identified wherein the autophagy machinery is recruited to pathogen-containing phagosomes, termed MAPLC3A (LC3)-associated phagocytosis (LAP), which results in optimal degradation of the phagocytosed cargo. Here, we describe the engageme...

Blood has been the most reliable body fluid commonly used for the diagnosis of diseases. Although there have been promising investigations for the development of novel lab-on-a-chip devices to utilize other body fluids such as urine and sweat samples in diagnosis, their stability remains a problem that limits the reliability and accuracy of readouts. Hence, accurate and quantitative separation ...

The present study investigated the biosorption capacity of live and dead cells of a novel Bacillus strain for chromium. The optimum biosorption condition was evaluated in various analytical parameters, including initial concentration of chromium, pH, and contact time. The Langmuir isotherm model showed an enhanced fit to the equilibrium data. Live and dead biomasses followed the monolayer bioso...

Necrosis refers to a spectrum of morphologic changes that follow cell death in living tissue, largely resulting from the progressive degradative action enzymes on lethally injured (cells placed immediately fixative are dead but not necrotic).

This article illuminates the transmedia storytelling techniques in The Walking Dead comic book and video game. Telltale Games’ The Walking Dead localizes itself within the comic book world of The Walking Dead by acting as a transmedia storytelling device and using intertextuality comics to assist game players with meaning-making. By participating in the game, Telltale rewards players with addit...

 Recently, special type of fuel cells has been developed that operates in a Dead End mode. Working in this condition, the Dead-End fuel cell is supplied withhydrogen almost at the same rate of consumption. It is noteworthy to mention thatall of the water transport mechanisms have been considered in this simulation.Water accumulation that is directly proportional to the cell current is of a grea...