Metallothionein (MT) is important because it binds tightly to heavy metals to decrease their toxicity. DNA was isolated from 30 toxic metal exposed and 30 toxic metal unexposed Zebu cows. The amplified metallothionein isoform-2 (MT-2) PCR product (489 bp) was further used for PCR-RFLP and DNA sequencing. MT-2 TaqI PCR-RFLP revealed homozygous genotype (AA) except for the E23 animal (AB). The ge...

Nitrogen (N) in plants is generally diagnosed by a soil test and plant tissue analysis.However, such analyses are costly in terms of time and money and are not easily accessible byresearchers and extension workers, let alone farmers. Alternative cost-effective methods arerequired for rapid analysis of the N status of crops and to guide N management in wheat. Theobjective of this study was to as...

The enormous growth of the Internet has confronted nearly every agent operating in the Internet, human or otherwise, with documents written in unknown languages. In order to allow unrestricted access to these data, the availability of language processing tools within the Internet is a prerequisite. Furthermore, it will be absolutely necessary to interconnect these tools given the almost unlimit...

Introduction: This study evaluated the effects of pretreatments with melatonin (MT), and Alpha Lipoic acid (ALA) on lopinavir/ritonavir (LPV/r) -induced serum levels of creatinine (Cr), urea (U), uric acid (Ua) and kidney levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT) in male albino rats. Effects of treatments with MT and ALA were also evaluate...

Diploid clones homozygous (mt(+)/mt(+) or mt(-)/mt(-)) or heterozygous (mt(+)/mt(-); phenotypically mt(-)) for the mating-type locus and homoplasmic for a chloroplast marker conferring resistance to an antibiotic were isolated by artificially induced cell fusion or sexual mating. These diploids were crossed with haploid or diploid strains of opposite mating type and carrying another chloroplast...