Eleven short tandem repeat loci (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, TH01, and TPOX) were investigated in 232 individuals from 6 Amazonian native tribes (Tiriyó, Waiãpi, Zoé, Urubu-Kaapor, Awa-Guajá, and Parakanã). We added the new data to a database that included five previously typed native populations from the same area (Wai Wai, Gavião, Zoró, Suruí, a...

The ability to identify individual human hosts based on analyses of blood recovered from hematophagous insects is beneficial for both medical and forensic entomology. Bed bugs, Cimex lectularius L. (Heteroptera: Cimicidae), may have several advantages over other blood-feeding arthropods for forensics because they do not remain on the host after their blood-feeding activity and remain in close p...

In the case of mass disasters, missing persons and forensic caseworks, highly degraded biological samples are often encountered. It can be a challenge to analyze and interpret the DNA profiles from these samples. Here we provide a new strategy to solve the problem by taking advantage of the intrinsic structural properties of DNA. We have assessed the in vivo positions of more than 35 million pu...

PURPOSE The aim of the study was assessment of environmental effect on typeability of AmpFISTR SGM Plus loci: D3S1358, VWA, D16S539, D2S1338, D81179, D21S11, D18S51, D19S433, TH01, FGA and gender marker amelogenin. MATERIAL AND METHODS Kidney, liver, spleen and pancreas tissue specimens collected during autopsies of five persons aged 20-30 years were incubated at 21 degrees C and 4 degrees C ...

PURPOSE Head and neck cancers account for about 6% of all human cancers. Molecular changes leading to the disease development and progression still remain not fully explained. Examination of loss of heterozygosity (allelic loss, LOH) using the specific microsatellite markers is a method of choice in assessing tumour suppressor genes (TSGs) localisation in human genome. MATERIAL AND METHODS Th...

Investigations of any type of crime invariably starts at the crime scene by collecting evidence. Thus, the purpose of this research was to collect and analyze an entomological trace from an environment that is similar to those of indoor crime scenes. Hematophagous mosquitoes were collected from two residential units; saliva of volunteers that were residents in the units was also collected for g...

Ever since the inception of STR-based forensic DNA analysis for human identification and profiling, approach developed continuously. Most these developments are pertaining to achieve set best polymorphic loci analysis, high inhibitor tolerance capability, increase in sensitivity, reduction turnaround time, cost-effectiveness. On similar line, SF25™ PCR amplification kit, a 6 fluorescent dye-che...

AIM To establish allele frequencies and genetic parameters for 5 new European Standard Set short tandem repeat (STR) loci in the population of Romania and to compare them with those in other populations. METHODS DNA was isolated using QIAamp 96 DNA Swab BioRobot Kit and Chelex 100 methods. Polymerase chain reaction amplification was done using Investigator ESSplexPlus Kit (D1S1656, D2S441, D2...

In this study we reported the results for the first time of applying Polymerase Chain Reaction-Short Tandem Repeats (PCR-STR) method in the field of detection of aneuploidies for chromosomes 21 and 18 in Croatians. The aims of the study were: (I) validation of the diagnostic informativeness of 6 STR loci (D18S51, D18S858, D18S535, D21S1435, D21S1411, and D21S1414) in sample of 205 unrelated hea...

Analysis of length polymorphism at short tandem repeat (STR) loci utilizing the polymerase chain reaction (PCR) process has proven to be an ideal assay for human identification purposes. The short length of STR loci coupled with the amplification of target sequence through PCR allows for a robust, sensitive, and specific assay for highly polymorphic markers. A multiplex containing fifteen STR l...