BACKGROUND A stable-isotope dilution method for the sensitive determination of D-galactose in human plasma was established. METHODS D-[(13)C]Galactose was added to plasma, and the concentration was measured after D-glucose was removed from the plasma by treatment with D-glucose oxidase and the sample was purified by ion-exchange chromatography. For gas chromatographic-mass spectrometric analy...

Lipopolysaccharide (LPS) is essential for most gram-negative bacteria and plays an important role in serum resistance, pathogenesis, drug protection from harsh environments. The outer core oligosaccharide of LPS involved bacterial recognition invasion host cells. D-galactosyltransferase WaaB responsible the addition D-galactose to LPS, which Salmonella typhimurium invasion. Here we report first...

BACKGROUND/AIMS Previous studies from our laboratory have revealed impaired intestinal absorption of D-galactose in lipopolysaccharide-treated rabbits. The aim of the present work was to examine the effect of LPS on D-galactose intestinal absorption in vitro. METHODS D-galactose intestinal transport was assessed employing three techniques: sugar uptake in rings of everted jejunum, transepithe...

Animal models are commonly used in brain ageing research. Amongst these, models where rodents are exposed to d-galactose are held to recapitulate a number of features of ageing including neurobehavioral and neurochemical changes. However, results from animal studies are often inconsistent. To better understand the characteristics of the model and effects of d-galactose on neurobehavioral and ne...

Using molecular dynamics (MD) simulation methods, we attempted to explain the experimental results on ligand specificity of glucose/galactose-binding protein (GGBP) to β-D-glucose and β-D-galactose. For the simulation, a three-dimensional structure of GGBP was prepared, and homology modeling was performed to generate variant structures of GGBP with mutations at Asp14. Then, docking was carried ...

Background: High content of phospholipids in testes and epididymis is involved in regulation of spermatogenesis. On the other side, it makes testes susceptible to oxidative stress due to lipid peroxidation, which alters the normal mechanism of spermatogenesis. Objective: In the present investigation, antioxidant effects of ethanolic extracts of parsley, lettuce and brahmi were tested against D-...

Paucilactobacillus wasatchensis, a nonstarter lactic acid bacteria, can cause late gas production and splits cracks in aging cheese when it metabolizes 6-carbon substrates, particularly galactose, to 5-carbon sugar, resulting the release of CO2. Previous studies have not explained no galactose is present. Based on genome sequence Pa. wasatchensis WDC04, genes for potential metabolic pathways we...

In the present study, we examined the effects of Dendropanax morbifera Léveille leaf extract (DML) on D-galactose-induced morphological changes in microglia and cytokines, including pro-inflammatory cytokines (interleukin [IL]-1β, IL-6, and tumor necrosis factor [TNF]-α) and anti-inflammatory cytokines (IL-4 and IL-10) in the hippocampus. Administration of DML to D-galactose-treated mice signif...