Cytochrome P-450 was purified to a specific content of 16 nmol/mg of protein from bovine adrenal cortex microsomes by hydrophobic chromatography using an w-amino-n-octyl Sepharose column in the presence of Emulgen 913, a nonionic detergent. The purified preparation exhibited a single polypeptide band (Mr = 47,000 f 1,000) when submitted to sodium dodecyl sulfate-polyacrylamide gel electrophor...

Depending on the amount of aeolian sediment input and dune erosion, dune size and morphology change over time. Since coastal foredunes play an important role in the Dutch coastal defence, it is important to have good insight in the main factors that control these changes. In this paper the temporal variations in foredune erosion and accretion were studied in relation to proxies for aeolian tran...

Many industries with avoidable fixed costs face competitive price instability problems in an attempt to maintain profitability. We designed an experimental environment where profitability is eroded by the addition of sunk and avoidable fixed costs. While we cannot reject the hypothesis that sunk fixed costs have no effect on prices, we observe a pattern of price signaling and responses which ma...

Accurate prediction of erosion thickness is essential for pipe engineering. The objective of the present paper is to study the temperature distribution in an eroded bend pipe and find a new method to predict the erosion reduced thickness. Computational fluid dynamic (CFD) simulations with FLUENT software are carried out to investigate the temperature field. And effects of oil inlet rate, oil in...

List of papers The present thesis is based on the following papers, which will be referred to by their Roman numerals.

it has been proposed that organic nitrates such as glyceryl trinitrate (gtn), used in the treatment of cardiovascular diseases, act by producing nitric oxide (no). however, the biochemical pathway for no formation from gtn is not well understood. in the present study, we showed that nitrate formation from gtn, by isolated rat hepatocytes, was inhibited about 50% when cellular glutathione was de...

The 7a-hydroxylation of taurodeoxycholic acid and the 6&hydroxylation of taurochenodeoxycholic acid and lithocholic acid were studied with a reconstituted system from rat liver microsomes consisting of partially purified cytochrome P-450, NADPH-cytochrome P-450 reductase, a synthetic phosphatidylcholine and NADPH or an NADPH-generating system. 7a-Hydroxylase activity was observed with cytochrom...

The administration of 3-methylcholanthrene to rats leads to changes in the enzyme systems of the liver which oxidize drugs. These changes have been related to changes in some of the spectral properties of microsomal cytochrome P-450. The altered cytochrome appearing after 3-methylcholanthrene treatment has been called P,-450,’ P-448, P-446, Pa450, and high-spin P-450 by different investigators....