These investigations are part of an attempt to study and interpret the intermediary metabolism of the kidneys in experimental renal hypertension. Hypertension was produced in dogs by the clamping procedure of Goldblatt and associates or by the silk perinephritis method of Page. Enzymatic studies were made by means of Warburg's manometric method. Cytochrome c was in addition determined spectroph...

1. The assembly of rat liver cytochrome oxidase was studied in isolated hepatocytes and isolated liver mitochondria labelled with L-[35S]methionine. 2. Labelled subunits II and III appeared in the immunoabsorbed holoenzyme within minutes after the initiation of a pulse label. In contrast, labelled subunit I appeared in immunoabsorbed holoenzyme only after a subsequent 2 h chase or after an addi...

In Papers I and II (1, 2) it was shown that basic phenylmercuric nitrate depressed a number of enzymes (cytochrome oxidase, succinoxidase, catalase, and several dehydrogenases), that it reacted with sulfhydryl groups, and that the depression of yeast respiration by the basic nitrate could be prevented by the presence of sulfhydryl-containing compounds, such as cysteine or homocysteine. These fi...

An important role in ensuring the functioning of respiratory chain belongs to cytochrome part, which includes complexes III (ubiquinol-cytochrome c oxidoreductase) and IV (cytochrome oxidase). The key components these enzymatic are heme-containing cytochromes, number depends on balance heme synthesis catabolism. δ-Aminolevulinate synthase catalyzes first step biosynthetic pathway, while oxygena...

A study of the mealybug genus Planococcus Ferris, 1950 (Hemiptera, Coccomorpha, Pseudococcidae) known from China is presented and 12 species are recognised. Of these, camelliae Zhang, sp. nov. described as new to science based on morphology adult female, P. bambusifolii (Takahashi, 1951) recorded for first time. Molecular analyses mitochondrial gene cytochrome c oxidase subunit I (COI) a key in...

Since October 2019, chinch bugs have been detected in large sunken dead patches of St. Augustinegrass (Stenotaphrum secundatum) lawns different locations Lisbon and Setubal districts, Portugal. Based on morphological molecular studies, using mitochondrial Cytochrome C Oxidase subunit I (COI) gene, the collected insects were identified as Blissus insularis (Hemiptera, Heteroptera, Blissidae). Th...

The influence of protein phosphorylation on the kinetics of cytochrome c oxidase was investigated by applying Western blotting, mass spectrometry, and kinetic measurements with an oxygen electrode. The isolated enzyme from bovine heart exhibited serine, threonine, and/or tyrosine phosphorylation in various subunits, except subunit I, by using phosphoamino acid-specific antibodies. The kinetics ...

The identification of the efficiency of some mtDNA genes of Mus musculus species complex (house mouse) for biosystematics research was studied in this approach. Recent studies have made use of different mitochondrial genes including NADH dehydrogenase genes, cytochrome b gene, cytochrome oxidase genes, D-loop region and whole mtDNA genome to study the house mouse species. Usage of each of these...

A new species of the monotypic genus Weygoldtia Miranda, Giupponi, Prendini & Scharff, 2018 was described from Con Son island, southern Vietnam, namely condao sp. nov. The is clearly distinguished its congener davidovi (Fage, 1946) by having more articles in tibia and tarsus leg I. fragment cytochrome c oxidase subunit I (COI) gene also provided for species. K2P genetic distance COI between...

BACKGROUND Cytochrome oxidase IV complex regulates energy production in mitochondria. Therefore, we determined the relation of COX genes with atherosclerosis in mice and pigs. METHODS AND RESULTS First, we compared atherosclerosis in the aortic arch of age-matched (24 weeks) C57BL/6J control (n = 10), LDL-receptor deficient (n = 8), leptin-deficient ob/ob (n = 10), and double knock-out (lacki...