1. Mitochondria isolated from rat liver were disrupted with 0.3 per cent deoxycholate and a number of subfractions were isolated from this preparation by differential centrifugation. 2. The protein N, RNA and phospholipide content, as well as the succinoxidase, cytochrome c oxidase, adenylate kinase, and DPNH-cytochrome c reductase of these fractions were determined. 3. Two of these subfraction...

Rapid separation of avian heterophils from anticoagulated whole blood was achieved using Ficoll-Hypaque discontinuous gradients. An average of 14.4% of blood heterophils was harvested with a mean purity exceeding 99%. Heterophil viability, as determined by trypan blue dye exclusion, averaged 99.8%. The integrity of isolated heterophils was evaluated by cytochemical staining and ultrastructural ...

WALTER J. FRAJOLA T HE EFFECTS of x-radiat.ion on the chemical constituents of cells of the l)ody are not well understood. Lymphoid tissue appears to l)e particularly sensitive to x-radiation,’’ nitrogen mustard,6’7 and triethylene melamine.7 Aminopteriis produces relatively little change in lymphoid tissue, hut. injures myeloid tissue severely.8’#{176} Few cytochemical studies of the effect of...

The cytochemical localization of G6Pase activity, which is specific to the endoplasmic reticulum (ER) of Müller cells, was studied after argon laser photocoagulation in the guinea pig retina. After argon laser radiation, Müller cells exhibited enlargement of the cytoplasm, an increase of reactive ER and the nuclei, dislocation of the nuclei and diagonal stretching of the cytoplasm. However, cel...

Proper fixation of lung tissue for morphological and cytochemical studies by transmission electron microscopy (TEM) is difficult by conventional (perfusion and immersion) fixation processes. A broiler model (Gallus domesticus) of pulmonary hypertension was the source of lungs for this study. Microwave and intermittent vacuum were used in order to have better structural and enzymatic preservatio...

A new cytochemical technique, sensitive to altered lysosomal membrane permeability of blood neutrophils, has been evaluated as a screening test for bacterial infection. This technique, for the lysosomal enzymes acid phosphatase and chloroacetate esterase, was compared with the neutrophil alkaline phosphatase and nitroblue tetrazolium tests. The mean score for each method was significantly highe...