THIS CASE OF PROTRACTED diarrhoea in an Australian traveller initially appeared consistent with coeliac disease. Further analysis revealed infection with Cyclospora cayetanensis, which was successfully treated with antibiotics. The increasing number of documented outbreaks of Cyclospora infection,1 and the need for special preparation of stool samples for their detection,2 highlight the importa...

To the Editor: In the article by N.J. Pieniazek and B.L. Herwaldt (1) on the rRNA gene of Cyclospora cayetanensis, the authors suggest that Cyclospora should be placed in the genus Eimeria because the rRNA genes of the two genera have similar sequences. The article refers to Norman D. Levine’s chapter on the Apicomplexa in the Illustrated Guide to the Protozoa (2). Regrettably, the authors fail...

This study aimed to develop a multiplex-touchdown PCR method to simultaneously detect 3 species of protozoan parasites, i.e., Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the major causes of traveler's diarrhea and are resistant to standard antimicrobial treatments. The target genes included the Cryptosporidium oocyst wall protein for C. parvum, Glutamate dehydrogenase ...

Cryptosporidium and Cyclospora are obligate, intracellular, coccidian protozoan parasites that infest the gastrointestinal tract of humans and animals causing severe diarrhea illness. In this paper, we present an overview of the conventional and more novel techniques that are currently available to detect Cryptosporidium and Cyclospora in water. Conventional techniques and new immunological and...

Cyclospora cayetanensis is an apicomplexan protozoan parasite which has emerged as an important cause of epidemic and endemic diarrhea. Water-borne as well as food-borne outbreaks have occurred, including a large number of U.S. cases associated with raspberries imported from Guatemala. Molecular markers exist for tracing the epidemiology of many of the bacterial pathogens associated with water-...

An outbreak of cyclosporiasis occurred in attendees of a wedding reception held in Philadelphia, Pennsylvania, on June 10, 2000. In a retrospective cohort study, 54 (68.4%) of the 79 interviewed guests and members of the wedding party met the case definition. The wedding cake, which had a cream filling that included raspberries, was the food item most strongly associated with illness (multivari...

Electrorotation is a noninvasive technique that is capable of detecting changes in the morphology and physicochemical properties of microorganisms. Electrorotation studies are reported for two intestinal parasites, Giardia intestinalis and Cyclospora cayetanensis. It is concluded that viable and nonviable G. intestinalis cysts can be differentiated by this technique, and support for this conclu...