We applied single-molecule photobleaching to investigate the stoichiometry of human Orai1 and Orai3 channels tagged with eGFP and expressed in mammalian cells. Orai1 was detected predominantly as dimers under resting conditions and as tetramers when coexpressed with C-STIM1 to activate Ca(2+) influx. Orai1 was also found to be tetrameric when coexpressed with STIM1 and evaluated following fixat...

Recursive spectral bisection (RSB) is a heuristic technique for finding a minimum cut graph bisection. To use this method the second eigenvector of the Laplacian of the graph is computed and from it a bisection is obtained. The most common method is to use the median of the components of the second eigenvector to induce a bisection. We prove here that this median cut method is optimal in the se...

maximizing economic earnings is the most common goal in cut-off grade optimization of open-pit mining operations. when this is the case, the price of the product has a critical effect on optimum value of cut-off grade. this paper investigates the relationship between optimum cut-off grade and price to maximize total cash flow and net percent value (npv) of operation. in order to visualize this ...

in this paper, we study an equivalence relation on the set of fuzzysubgroups of an arbitrary group g and give four equivalent conditions each ofwhich characterizes this relation. we demonstrate that with this equivalencerelation each equivalence class constitutes a lattice under the ordering of fuzzy setinclusion. moreover, we study the behavior of these equivalence classes under theaction of a...

The max-k-cut problem is to partition the vertices of a weighted graph G = (V, E) into k ≥ 2 disjoint subsets such that the weight sum of the edges crossing the different subsets is maximized. The problem is referred as the max-cut problem when k = 2. In this work, we present a multiple operator heuristic (MOH) for the general max-k-cut problem. MOH employs five distinct search operators organi...

the effects of sodium chloride (nacl) salinity (control; canal water with 0.4 ds m-1), 2.5 dsm-1, 5.0 ds m-1, 7.5 ds m-1 and 10.0 ds m-1, respectively) developed after 6 weeks of pruning (beginning of study) on plant growth, flowering and quality of three cut rose (rosa hybrida l.) cultivars viz. ‘kardinal’, ‘angelique’ and ‘gold medal’ were studied to achieve better management, quality product...

the notion of strong arcs in a fuzzy graph was introduced bybhutani and rosenfeld in [1] and fuzzy end nodes in the subsequent paper[2] using the concept of strong arcs. in mordeson and yao [7], the notion of“degrees” for concepts fuzzified from graph theory were defined and studied.in this note, we discuss degrees for fuzzy end nodes and study further someproperties of fuzzy end nodes and fuzz...