نتایج جستجو برای: culture filtrate
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Phellinus linteus, a natural growing mushroom, has been known to exhibit anti-tumor, anti-inflammatory, anti-allergic and anti-oxidant effects. Aiming to exploit the neuroprotective effects of P. linteus, we evaluated its effects on infarct volume reduction in a rat model of focal cerebral ischemia. Male Sprague-Dawley rats were subjected to right middle cerebral artery occlusion. Filtrate of P...
Hedgecock, Loyd W. (Veterans Administration Hsopital, St. Louis, Mo.). Resistance-enhancing activity of culture filtrates of Mycobacterium tuberculosis. J. Bacteriol. 88:1349-1355. 1964.-It was demonstrated that filtrates of cultures of Mycobacterium tuberculosis exhibit resistance-enhancing activity against infection with Klebsiella pneumoniae and Diplococcus pneumoniae. Resistance was demonst...
A culture filtrate of Bacillus circulans KA-304 grown on a cell-wall preparation of Schizophyllum commune has an activity to form protoplasts from S. commune mycelia. alpha-1,3-Glucanase and chitinase I, which were isolated from the filtrate, did not form the protoplast by itself while a mixture of them showed protoplast-forming activity. Streptomyces cyaneus SP-27 was isolated based on the pro...
It was previously postulated, based on indirect evidence, that Clostridium botulinum type B produces neurotoxin which is initially of low toxicity but which then becomes activated to highly toxic form by the action of an endogenous enzyme(s). The first direct in vitro experimental evidence in support of this hypothesis is presented here. The mildly active toxin (progenitor toxin) produced by C....
containing 30 liters of a medium with agitation rate of 400rpm and air flow of 30 liters/minute. The medium consisted of glucose 2.5%, soybean meal 1.5% dry yeast 0.2% and CaCO3 0.4%; pH was adjusted to 6.2. After fermentation for 90 hours, the culture broth was separated into filtrate and mycelium by centrifugation. The flow diagram for the isolation of I is shown in Fig. 1. The culture filtra...
Culture filtrate proteins (CFPs) of M. bovis were produced by culturing the organism for a period of 3 weeks. These CFPs elicited a good delayed hypersensitivity (DTH) reaction in cattle and guinea pig. However, CFPs showed least cross-reactivity when tested in guinea pigs sensitized with M. bovis BCG and M. phlei. CFPs were found to be well recognized by T-cells of single intradermal test (SID...
Allergic bronchopulmonary aspergillosis (ABPA) is the result of hypersensitivity to Aspergillus antigens in patients with long-standing atopic asthma. In the present study mycelial and culture filtrate antigens from Aspergillus fumigatus cultures isolated from diverse sources were tested against sera of 10 ABPA patients and 10 control individuals by an ELISA methodology. The results indicate hi...
Aphids are one of the most destructive pests in crop production such as pepper, cucumber, and eggplants. The importance of entomopathogenic fungi as alternative pest control agents is increasing. Conidia of entomopathogenic fungi are influenced by environmental conditions, such as temperature and relative humidity, and cause slow and fluctuating mortality. These factors have prevented wider app...
We have examined the effect of complete cell recycle on the production of cholera toxin (CT) by Vibrio cholerae and CT-like toxin by Vibrio mimicus in continuous culture fermentations. Complete cell recycle was obtained by filtering culture fluids through Amicon hollow fibers with an exclusion limit of 100,000 daltons (H1P100-20) and returning the concentrated cell slurry to the fermentor. A si...
A soil microorganism producing αand β-glucosidase inhibitors was identified as Bacillus lentimorbus, based on the fatty acid and morphological analyses, along with biochemical and physiological tests. The α-glucosidase inhibitor was highly produced by this strain in a culture medium containing 0.25% of sodium glutamate and 0.5% of glucose, pH 8.0 at 30C for 2 days. The α-glucosidase inhibitor f...
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