Transmission experiments of different isolates of Cucumber mosaic virus (CMV), Watermelon mosaic virus (WMV) and Zucchini yellow mosaic virus (ZYMV) were conducted using established colonies of Aphis gossypii Glover, Myzus persicae (Sulzer), A. fabae Scopoli and A. craccivora Koch as vectors. The transmission procedure used provided an estimate of the virus transmission efficiency for each aphi...

Artificial microRNAs (amiRNAs) are the expression products of engineered microRNA (miRNA) genes that efficiently and specifically downregulate RNAs that contain complementary sequences. Transgenic plants expressing high levels of one or more amiRNAs targeting particular sequences in the genomes of some RNA viruses have shown specific resistance to the corresponding virus. This is the case of th...

This communication describes the in vitro assembly of genetically recombinant Cucumber Mosaic Virus (CMV) viral capsid proteins (CPs) into biological nanotubes, several micrometres long yet with a diameter of only approximately 17 nm, triggered by double-stranded DNAs of different lengths.

s of conference presentations/posters NEMES K, GELLÉRT Á, BALÁZS E, SALÁNKI K (2014) Az uborka mozaik vírus (Cucumber mosaic virus, CMV) 2b fehérjéjének szerepe a vírus növényen belüli terjedésében független a géncsendesítés szuppresszálásától 60. Növényvédelmi Tudományos Napok. Budapest 2014. február 18-19

We defined the minimal core promoter sequences responsible for efficient and accurate initiation of cucumber mosaic virus (CMV) subgenomic RNA4. The necessary sequence maps to positions -28 to +15 relative to the initiation cytidylate used to initiate RNA synthesis in vivo. Positions -28 to -5 contain a 9-bp stem and a 6-nucleotide purine-rich loop. Considerable changes in the stem and the loop...

Virus diseases greatly affect oilseed rape (Brassica napus) production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antiviral gene) with different genus viruses by constructing BnSGS3-overexpressing (BnSGS3-Ov) and BnSGS...

A new method of inoculation of gladiolus with cucumber mosaic virus (CMV) was developed using the Bio-Rad Helios Gene Gun System. This method circumvents the traditional use of aphids to transmit CMV, a virus that is mechanically transmissible to many plant species but only with difficulty to gladiolus. Cartridges containing virus-coated gold microcarriers were prepared and the virus shot into ...

Transgenic tobacco showing replicase-mediated resistance against cucumber mosaic virus (CMV) can be infected by the strain K-CMV. By use of chimeric constructs between full-length cDNA clones of RNA2 of strains Fny-CMV and K-CMV, the existence of two independent mechanisms of replicase-mediated resistance against viral replication and movement of Fny-CMV was demonstrated in these plants. The da...

association of satellite rna with cucumber mosaic virus (cmv) leads to either attenuation or enhancement of the viral symptoms. for detection of the satellite rnas in iranian isolates of cmv, plant samples showing viral symptoms were collected from different farms in the provinces of east- and west- azerbayjan and ardebil. these samples were inoculated onto the experimental host plants in green...