Amongst the recognized classes of naturally occurring antimicrobials, human host defense peptides are an important group with an advantage (given their source) that they should be readily translatable to medicinal products. It is also plausible that truncated versions will display some of the biological activities of the parent peptide, with the benefit that they are less costly to synthesize u...

One of the most important virulence factors in Pseudomonas aeruginosa is biofilm formation, as it works a barrier for entering antibiotics into bacterial cell. Different environmental and nutritional conditions were used to optimize formation using microtitre plate assay by P. aeruginosa. The low nutrient level medium represented tryptic soy broth (TSB) was better than high with Luria Broth (LB...

Results with two ultramicro methods for plasma uric acid analysis with uricase are described: a crystal violet (gentian violet) method, modified from an automated procedure by Gochman and Schmitz [C/in. Chem. 17, 1154 (1971)] and adapted to manual analysis, and a polarographic oxygen-sensing system (Beckman Instruments,Inc.).The coefficientof variation (CV) for the crystal violet method varied ...

Clonogenic assay or colony formation assay is an in vitro cell survival assay based on the ability of a single cell to grow into a colony. The colony is defined to consist of at least 50 cells. The assay essentially tests every cell in the population for its ability to undergo ‘‘unlimited’’ division. Clonogenic assay is the method of choice to determine cell reproductive death after treatment w...

Pseudomonas aeruginosa is a multi-drug resistant (MDR) human pathogen and known for their biofilm growth making it difficult to eliminate. This research aims determine the effectiveness of water extract melinjo leaf (Gnetum gnemon L) on inhibition cell attachment, degradation using Crystal Violet (CV) Assay. Reasearch results showed that contains saponin, alkaloid, tanin dan steroidal antibacte...

This study investigated the potential biological activities by determining antioxidant (DPPH Assay), antibacterial (Agar-well Diffusion Method), and antiquorum sensing (Crystal Violet based Microtitre Plate Biofilm Assay) of selected wild tea leaves Mt. Province, namely: Cinnamomum mercadoi S. Vidal, Gaultheria leucocarpa var. cumingiana (S.Vidal), Clausena sanki (Perr.) J.F. Molino mollis (Mer...

Honey is a rich source of carbohydrates, while minor compounds such as amino acids and polyphenols contribute to its health-promoting effects. one the oldest traditional remedies applied for microbial infections, due antibacterial, anti-inflammatory, antioxidant properties. The aim this study was investigate antibacterial antibiofilm effects Hungarian black locust, linden, sunflower honeys agai...

In this work, we used a simple and totally green method for synthesizing silver nanoparticles using kappa-carrageenan as reducing and stabilizing agent. The beads were prepared in aqueous medium by microwave heating, and then followed by cross-linking with K+ cations without using any additional toxic and expensive chemical agents. The preparation method of the carrageenan-based beads is easy, ...

The present work was performed to determine the impacts of commercial disinfectants against biomass, viability, and biochemical composition Salmonella enterica serovar Thyphimurium ATCC14028 biofilm. biofilm grown in microplates exposed namely sodium hypochlorite, benzalkonium chloride, chloroxylenol, dodecyl-benzene sulfonate-based disinfectants. Biofilm were determined using crystal violet as...

BACKGROUND Achillea kellalensis, which is frequently used by Chaharmahal va Bakhtiarians residing in, Southwest of Iran, as a traditional herbal medicine for the treatment of acute diarrhea, has been selected to examine its antiviral activities against bovine rotavirus and cell toxicity activity in MA-104 cells. OBJECTIVES The aim of this study was to evaluate the in vitro cytotoxic and anti-...