Cryo-electron tomography of vitreous cryo-sections is the most suitable method for exploring the 3D organization of biological samples that are too large to be imaged in an intact state. Producing good quality vitreous cryo-sections, however, is challenging. Here, we focused on the major obstacles to success: contamination in and around the microtome, and attachment of the ribbon of sections to...

AIMS Within the last several years, transvenous cryo-ablation has become an alternative method to perform ablation of the slow-pathway. This study evaluated the acute and long-term safety and effectiveness of atrio-ventricular nodal re-entrant tachycardia (AVNRT) cryo-ablation. METHODS AND RESULTS The first 69 consecutive patients with AVNRT (60 slow-fast, 4 fast-slow, and 5 slow-slow) who un...

The purpose of this study was to investigate the immediate and long-term results of cryoplasty versus conventional balloon angioplasty in the femoropopliteal artery of diabetic patients. Fifty diabetic patients (41 men, mean age 68 years) were randomized to cryoplasty (group CRYO; 24 patients with 31 lesions) or conventional balloon angioplasty (group COBA; 26 patients with 34 lesions) of the f...

Abstract Animals, plants, and fungi live in a microbe-dominated world. Investigating the interactions processes at host-microbe interface offers insight to how bacteria influence development, health, disease of host. Optimization existing imaging technologies development novel instrumentation will provide tools needed fully understand dynamic relationship that occurs throughout lifetime In this...

Electron Cryo-microscopy (cryo-EM) is an important biophysical technique that produces 3dimensional (3D) images at different resolutions. De novo folding is becoming a promising approach to derive the backbone of proteins from the cryo-EM 3D images at medium resolutions. We summarize our findings in major steps of de novo folding and the challenges from the

Cryo-electron microscopy has seen incredible progress recently. From the first reconstruction of frozen hydrated samples to recent atomicresolution structures, structural biology is undergoing a revolution. However, until recently, the resolution of cryo-EM structures was often significantly lower than that of X-ray crystallography. We have reached a point where, for an increasing number of sam...

Cryo-electron microscopy (cryo-EM) is a structural molecular and cellular biology technique that has experienced major advances in recent years. Technological developments in image recording as well as in processing software make it possible to obtain three-dimensional reconstructions of macromolecular assemblies at near-atomic resolution that were formerly obtained only by X-ray crystallograph...

BACKGROUND Transbronchial biopsy (TBB) which is performed with metal forceps (forceps TBB) has been accepted as a useful technique in establishing diagnoses of diffuse lung diseases (DLDs). The use of cryoprobes to obtain alveolar tissue (cryo-TBB) is a new method which is currently used by our institute as well as others with excellent results. OBJECTIVES To assess the safety of cryo-TBB com...

OBJECTIVES The aim of this study was to evaluate the efficacy and safety of catheter-based cryo-therapy for septal tachycardia substrates in pediatric patients. BACKGROUND Cryo-therapy may be particularly useful for ablation of septal tachycardias, including atrioventricular nodal re-entry tachycardia (AVNRT), atrioventricular reciprocating tachycardia (AVRT), and ventricular tachycardia (VT)...