Identification of monokaryons and their mating types and discrimination of hybrid offspring are key steps for the crossbreeding of Pleurotus tuoliensis (Bailinggu). However, conventional crossbreeding methods are troublesome and time consuming. Using RNA-seq technology, we developed new expressed sequence tag-simple sequence repeat (EST-SSR) markers for Bailinggu to easily and rapidly identify ...

BACKGROUND Manufacture of MATa and MATα yeast cells is required for crossbreeding, a procedure that permits hybridization and the generation of new heterozygous strains. Crossbreeding also can be performed with a- and α-type of cells, which have the same mating abilities as MATa and MATα haploid cells, respectively. RESULTS In this work, we describe a method to generate a- and α-type of cells...

Genetic variation and differentiation at 32 microsatellite loci was quantified for parent-descendant cattle populations and parent-descendant bison () populations. We compared heterozygosity () and allelic richness () for 587 cattle of four breeds and three lines derived from them, and 188 bison in three pairs of parent-descendant populations. and were less in the Line 1 Hereford inbred cattle ...