Nanobiocatalysis, as the synergistic combination of nanotechnology and biocatalysis, is rapidly emerging as a new frontier of biotechnology. The use of immobilized enzymes in industrial applications often presents advantages over their soluble counterparts, mainly in view of stability, reusability and simpler operational processing. Because of their singular properties, such as biocompatibility...

Thermomyces lanouginosa lipase has been used to develop improved methods for carrier-free immobilization, the Cross-Linked Enzyme Aggregates (CLEAs), for its application in detergent products. An activator step has been introduced to the CLEAs preparation process with the addition of Tween 80 as activator molecule, in order to obtain a higher number of the individual lipase molecules in the ”op...

The industrial and environmental applications of laccase, especially in wastewater treatment, have gained focus recent years. Therefore, developing the proper laccase immobilization techniques, which could improve stability enzymes simplify required downstream processes, is needed. A novel two-step process was developed, resulting cross-linked enzyme aggregates (CLEA) pores membrane. Laccase ad...

?-galactosidase is an enzyme capable of hydrolysing lactose, used in various branches industry, mainly the food industry. As efficient industrial use enzymes depends on their reuse, it necessary to find effective method for immobilisation, maintaining high activity and stability. The present work proposes cross-linked magnetic aggregates (mCLEAs) prepare heterogeneous biocatalysts ?-galactosida...

background: timely diagnosis of leptospirosis is essential for early and effective treatment, for there are many differential diagnoses for it.. leptospiral researchers have an increasing interest in developing new serological methods with recombinant antigens to improve the leptospirosis diagnosis. several serological tests have been developed for the proper diagnosis of leptospirosis. objecti...

objectives: leptin is a hormone-like protein produced by the adipose tissue. it plays an important role in protection of host against inflammation and infection. some studies have reported changes in leptin levels in the gingival crevicular fluid (gcf), saliva and blood serum of patients with periodontal disease compared to healthy individuals. the aim of the present study was to compare the sa...

Enzymes are extensively used in biotechnological processes several areas of industry. They sustainable and safe, their specificity is another characteristic that improves the performance process. Among enzymes, lipase relevant due to ability play different roles industry possibility collecting them from microbial sources found industrial residues. This can reduce costs enzyme production. In rel...