Fibroblast growth factor-23 (FGF-23) is a phosphaturic hormone used to monitor chronic kidney disease (CKD) in humans. The aim of this pilot study was compare three diagnostic assays and assess how the results correlate with parameters renal dysfunction cats. Four groups 10 cats each were formed retrospectively according creatinine, based on IRIS staging. FGF-23 measured using two different ELI...

Second-order calibration and multivariate spectroscopic-kinetic measurements in the visible region are proposed to improve the Jaffé method for creatinine assay. Analyses performed on synthetic mixtures containing bilirubin, glucose, and albumin confirm that second-order calibration is useful for creatinine determination in human serum. Quantitative determinations of creatinine with the paralle...

We describe a procedure for specific, rapid, kinetic determination of creatinine, in which a manual coupled-enzyme micro-scale assay is adapted to a centrifugal analyzer. The creatinine reaction is ultimately linked to NADH utilization, which is measured by the absorbance change at 340 nm. This procedure requires 15 microL of serum and the standard curve is linear to a creatinine concentration ...

The effects of storage for 6 months or 2 years at -20 degrees C were studied in urine samples from Type II diabetic patients by assaying albumin by immunoturbidity, N-acetylglucosaminidase (EC 3.2.1.30) by methoxynitrovinylphenol release, and creatinine by the Jaffé method. There were significant decreases (P < 0.001) in albumin/creatinine ratios from 1.14 (0.63-2.98) to 0.83 (0.32-2.12) g/mol ...

The current work was done to determine the correlation between vitamin D and cystatin C as a predictor of kidney disease in males with diabetes mellitus. A total 60 were taken from October December 2018. They divided into 30 patients type 2 healthy volunteers' control group. Glycated hemoglobin HbA1c, plasma glucose level, creatinine urea measured for all subjects. Also, levels 25(OH) cystatin-...

Interference by cefoxitin with determination of creatinine is less with the Technicon SMAC than with other commercial analytical systems. The SMAC assay involves a single-point kinetic method with dialysis, whereas most other commercial methods are multipoint kinetic without dialysis. The apparent creatinine concentration measured for aqueous solutions of cefoxitin was 73 mmol of creatinine per...

Several cephalosporin antibioticscephalothin, cephaloglycine, cephaloridine, cephacetrile, and cefoxitinreportedly interfere positively with measurement of creatinine by the Jaff#{233} reaction. Others such as cefazolin, cephalexin, cephradine, cefamandole, and cephalpirin give negative or only very slight responses under the same in vitro conditions (1, 2). Rankin et al. (3) showed in vivo int...

An enzyme-linked immunosorbent assay (ELISA) for urinary retinol-binding protein (RBP) has been developed and compared with urinary beta 2-microglobulin for the detection of tubular proteinuria. The assay has a working range of 10 to 250 micrograms of RBP per liter of urine. The within-assay CV was 3.2-7.1%, the between-assay CV 12.5%. A control population of 118 male subjects gave a geometric ...

Introduction: Sickle cell nephropathy is a cause of significant morbidity and mortality in patients with sickle cell disease. Traditional creatinine based assay for evaluating renal function has several limitations. Hence, the need for more sensitive markers such as β2 microglobulin (β2M). This study seeks to compare β2M with creatinine as a marker of renal damage in patients with sickle cell d...