Coumarin analogues were synthezised and evaluated as acceptors for the intrinsic fluorescence resonance energy transfer (iFRET) of tryptophan residues in target proteins. The fluorescence properties such as quantum yields, iFRET efficiencies, and Förster distances of the prepared coumarin analogs were determined in a model system, by their conjugation to biotin, utilizing streptavidin (SAV) as ...

The synthesis of some fluorescent coumarin dyes derived from coumarin-3-carboxylic acid were described.The new dyes are obtained by condensation of coumarin – 3 carboxylic acid derivatives with para substituted ortho phenylenediamines and cyclisation of the mono condensed ortho phenylenediamines in an acid medium. The obtained dyes were characterized from their physico-chemical point of view by...

Headspace solid-phase microextraction (HS-SPME; PDMS/DVB fibre) and ultrasonic solvent extraction (USE; solvent A: pentane and diethyl ether (1:2 v/v), solvent B: dichloromethane) followed by gas chromatography and mass spectrometry (GC, GC-MS) were used for the analysis of Prunus mahaleb L. honey samples. Screening was focused toward chemical composition of natural organic volatiles to determi...

A series of novel thiazolidinones have been synthesized by reaction of various Schiff bases of coumarin with thioglycolic acid. The reaction of 4-hydroxy coumarin with POCl3 yielded 4chloro coumarin 2 and 4-chloro-3, 4', 3', 4"-tercoumarin 2a. Compound 2 was reacted with pphenylene diamine to yield 4-[(4-aminophenyl)amino]-2H-chromen-2-one.Various Schiff bases of coumarin were synthesized by co...

the synthesis of functionalized 2-oxo-2h-coumarin derivatives has been studied by a one-pot reaction of o-hydroxybenzaldehyde, ethyl 2-bromoacetate and triphenylphosphine in the presence of catalytic amount of triethyl amine in etoac, water or under solvent free conditions. we have found the best results obtained under solvent free condition.