The transcriptional regulator Cg1486 of Corynebacterium glutamicum ATCC 13032 is a member of the IclR protein family and belongs to the conserved set of regulatory proteins in corynebacteria. A defined deletion in the cg1486 gene, now designated ltbR (leucine and tryptophan biosynthesis regulator), led to the mutant strain C. glutamicum IB1486. According to whole-genome expression analysis by D...

There is growing evidence that the role of lipids in innate immunity is more important than previously realized. How lipids interact with bacteria to achieve a level of protection, however, is still poorly understood. To begin to address the mechanisms of antibacterial activity, we determined MICs and minimum bactericidal concentrations (MBCs) of lipids common to the skin and oral cavity--the s...

BACKGROUND Mastitis prevalence, milking procedures and management practices were investigated in 25 big dairy herds supplying milk to an Estonian dairy company. The aim of the study was to provide information for the company to be used in their new udder health improvement program to be set up after the completion of this study. METHODS Quarter milk samples were collected from 3,166 cows for ...

A total of 1,356 clinical isolates were tested against daptomycin by broth microdilution methods. Daptomycin was active against seven groups of viridans group streptococci (MIC50 and MIC90 values ranging from ≤0.06 and ≤0.06 μg/ml [Streptococcus bovis and Streptococcus dysgalactiae] to 0.5 and 1 μg/ml [Streptococcus mitis, Streptococcus oralis, and Streptococcus parasanguinis], respectively), b...

The fatty acid profiles of 74 strains of mycolic acid-containing coryneform bacteria were examined by gas-liquid chromatography. All of the strains contained major amounts of straight-chain and monounsaturated fatty acids although some also possessed substantial amounts of 10-methyloctadecanoic acid. Iso- and anteiso-branched acids were not present. Five distinct fatty acid patterns were eviden...

Between October 2005 and May 2006, a total of 727 badgers found dead in Wales were reported, and 550 were delivered to the Regional Laboratories of the Veterinary Laboratories Agency (VLA). Of the 459 carcasses suitable for examination, 55 were deemed to be infected with Mycobacterium bovis on the basis of culture, spoligotyping, and variable-number tandem repeat typing. Acid-fast bacteria were...

INTRODUCTION Among bacteria causing mastitis, only Streptococcus agalactiae, Staphylococcus aureus, Mycoplasma species, and Corynebacterium bovis are considered as fully contagious. Among these, S.aureus, is currently the most frequently isolated contagious pathogen in subclinical and chronic bovine mastitis worldwide (1). The reasons for the large spread of S.aureus intramammary infections (IM...

The first trial in the developmental phase of the "Performance Evaluation Program" based on the new programs of the International Council for Laboratory Animal Science (ICLAS) was carried out. ICLAS supplied test samples to each diagnostic laboratory for self-assessment of microbiological monitoring methods. We found that 1 mouse serum sample was positive for mouse minute virus and another was ...

The presence of pili in human and animal parasites and pathogens of the genus Corynebacterium was examined. C. kutscheri, C. diphtheriae, and C. pseudodiphtheriticum possessed a fairly large number of pili, ranging from dozens to more than a hundred, in 91 to 100% of the bacterial cells. C. equi, C. hoagii, C. xerosis, C. pyogenes, and C. murisepticum had only a small number of pili in 10 to 37...