The effects of fluorescein and Richardson's stain on corneal epithelial wound healing were compared in eyes of rabbits whose corneas had the epithelium removed by scraping or by n-heptanol. One eye of each rabbit was stained with fluorescein and the other eye was stained with Richardson's stain at intervals throughout the healing process, and the wounds were photographed for planimetry and dete...

Semifluorinated alkanes (SFA, e.g. perfluorobutylpentane F4H5, perfluorohexyloctane F6H8) are inert, non-toxic fluids capable of dissolving lipophilic drugs. The aim of this study to assess the bioavailability and safety of SFAs as drug solvents for the topical ocular application of Cyclosporin A (CsA). A commercially available CsA formulation (Restasis, 0.05% CsA in castor oil) was tested agai...

Proplast, a vitreous, carbon-Teflon, fluorocarbon polymer, was tested in rabbits for corneal tolerance and acceptance. Toxicity, vascularization, epithelialization, infection, and extrusion were studied clinically and histologically. Four techniques were used: implantation of irregular-shaped pieces of material in an interlamellar corneal pocket, lamellar graft implantation with one exposed sur...

Perfluorocarbon liquids (PFCLs) are useful and safe surgical tools in vitreoretinal surgery. The use of PFCL as a tamponade has been controversial due to the corneal toxicity, retinal infiltration, and inflammatory reaction in experimental studies. Several authors have studied in humans the anatomical and functional outcome and adverse effects of perfluorocarbon liquids used as short-, medium-,...

Amiodarone is an iodinated benzofuran derivative that represents a new and extremely effective therapy for certain life-threatening refractory cardiac arrgythmia. There are numerous side effects associated with amiodarone therapy, including corneal microdeposits. Skin reactions and others. Pulmonary toxicity, however, represents the most serious adverse reaction limiting the clinical effecacy o...

PURPOSE Topical preparations of cyclosporine (CsA) are common therapeutics for the treatment of dry eye. However, they are not devoid of side effects, such as allergy and irritation. The present study aimed at evaluating the safety profile of a new CsA formulation in cationic emulsion (CEm) in vitro with a dynamic corneal wound healing assay using human corneal epithelial (HCE) cells, and in vi...

BACKGROUND Rabbits are currently not a good model for studying diseases of the corneal endothelium because their corneal endothelial cells (CECs) maintain a high proliferative capacity throughout almost all their life. Addressing this particular feature might allow the use of this species for such a purpose. The aim of this study was to evaluate the corneal endothelial injury after intracameral...

BACKGROUND Limbal stem cell (LSC) deficiency leads to corneal opacity due to a conjunctivalization of the corneal surface. LSC transplantation, which can be followed by corneal keratoplasty, is an effective procedure to restore corneal transparency; however, a common cause of failure of this procedure is neovascularization (NV). METHODS A 59-year-old man with a 21-year history of a corneal ch...