Fifty-nine normal dogs, ranging in age from 6 weeks to 132 months were examined with contact specular microscopy to determine the relationship of age to corneal endothelial cell density, morphology, and corneal thickness. Canine corneal endothelial cells appear quite similar to those of other species studied, including man. The hexagonally shaped canine endothelial cells tend to enlarge with ag...

PURPOSE Extracellular matrices were tested for their ability to support the adhesion and proliferation of human corneal endothelial cells. METHODS Human corneal endothelial cells were plated onto tissue culture dishes coated with purified fibronectin or a matrix elaborated by cultured bovine corneal endothelial cells. The presence of human cells in the cultures was confirmed by karyotyping. C...

PURPOSE To describe the differences of corneal endothelial cell densities, cell size variability and cell hexagonality in cataract populations of south Asia between sexes and ethnic groups. METHODS 1,235 eyes of 1,235 male and female patients 40-75 years of age with senile cataract were examined with non contact specular microscopy with semi-automated analysis technique. The cell data of the ...

Twenty-one dogs were used to study the effects of phacoemulsification and extracapsular lens removal on corneal endothelium and corneal thickness. Specular microscopy revealed preoperative cell morphology and cell densities similar to man. After surgery endothelial cell counts significantly decreased 22% (central) and 13% (periphery) with phacoemulsification and 34% (central) and 31% (periphery...

BACKGROUND/AIM Corneal graft survival depends critically on the quality of the endothelium. In this study the authors aimed to evaluate corneal endothelium in mice at different times after transplantation and to correlate endothelial integrity with corneal graft survival. METHODS Syngeneic and allogeneic corneal grafts at various times (days 0-60) after engraftment were examined in flat mount...

PURPOSE Corneal edema is a significant component of the various forms of herpes simplex virus type 1 (HSV-1)-induced stromal disease. Maintenance of corneal thickness, a reflection of corneal hydration, depends on a physical barrier formed by endothelial cell-cell junctions and by the activity of Na+/K(+)-ATPase pumps that regulate ion flux and thus influence water movement through this cell la...

Diabetic keratopathy is an ocular complication that occurs with diabetes. In the present study, the effect of diabetic keratopathy on corneal optical density, central corneal thickness, and corneal endothelial cell count was investigated. One hundred and eighty diabetic patients (360 eyes) were enrolled in the study during the period from March, 2012 to March, 2013. The patients were divided in...

PURPOSE To compare the endothelial structure and thickness of the cornea in diabetic and nondiabetic patients, and to evaluate the systemic and ocular factors that contribute to the damage of endothelial cells in diabetic patients. METHODS The corneal endothelial structure and central corneal thickness (CCT) were investigated in 99 type II diabetic patients (99 eyes) and in 97 nondiabetic pat...

PURPOSE To determine the efficacy of the angiogenic inhibitor TNP-470 on inflammatory corneal neovascularization. Topical and systemic delivery of the drug were investigated in a murine model as well as inhibition of endothelial cell proliferation in vitro and in vivo. METHODS The effect of TNP-470 on VEGF- and bFGF-stimulated bovine capillary endothelial (BCE) cell proliferation was evaluate...

OBJECTIVE To evaluate the effects of phacoemulsification energy on the redox state and mitochondrial distribution of cultured human endothelial cells. METHODS Human corneal endothelial cells from fresh banked human donor tissue not suitable for transplantation were harvested and cultured. Cellular autofluorescence images were obtained using an inverted microscope. The redox fluorometric ratio...