Vibrio cholerae and Vibrio mimicus have similar phenotypes and genomes making rapid differentiation of these two species difficult. The first standard multilocus sequence analysis (MLSA) scheme for the identification of these species is described. A collection of 45 representative isolates from different geographical regions and hosts was examined using segments of the housekeeping genes pyrH, ...

Live eels and processed fish products from Malaysia are routinely checked for microbial pathogens before export to Japan. The eels and water from the ponds are screened for Vibrio cholerae and Salmonella spp, whereas the processed fish products are tested for microbial contamination (aerobic plate count), coliforms, E. coil and Vibrio cholerae. Results showed that live eels and water samples we...

cholera outbreak is more common in developing countries. the causative agent of the disease is vibrio cholerae strains o1 and o139. traditional diagnostic testing for vibrio is not always reliable, because vibrio can enter a viable but non cultivable state. therefore, nucleic acid-based tests have emerged as a useful alternative to traditional enrichment testing.       in this investigation, a ...

Cholera has been a prevalent disease worldwide since the early 19th century. Vibrio cholerae O1 and O139 are the two serogroups that have been mainly implicated in causing cholera. This study reports the results of biotyping, serotyping and phage typing of V. cholerae O1 and O139 (1998-2007) strains received from different parts of India for the identification of the trends in the occurrence an...

Vibrio parahaemolyticus and Vibrio cholerae are ubiquitous to estuarine and marine environments. These two species found in Mediterranean coastal systems can induce infections in humans. Environmental isolates of V. cholerae (n = 109) and V. parahaemolyticus (n = 89) sampled at different dates, stations and water salinities were investigated for virulence genes and by a multilocus sequence-base...

This is a retrospective study on Vibrio spp. isolated from patients with diarrhea (both inpatients and outpatients) at Siriraj Hospital, Bangkok, Thailand from January 1994 to December 2001. Of the 33,334 fecal specimens sent for culture, 2,229 (6.68%) were positive for pathogenic bacteria. The most commonly isolated bacteria was Vibrio (1,183 of 2,229 isolates, 53%). Of these 1,183 isolates, t...

The in vitro activity of norfloxacin and six other antimicrobial agents was tested against 93 vibrio strains representing the currently described pathogenic Vibrio species. Norfloxacin had excellent activity against all species, with the following MICs for 90% of the strains: 0.016 micrograms/ml for Vibrio cholerae (including tetracycline-resistant V. cholerae O1 strains), 0.25 micrograms/ml fo...

vibrio cholerae is a human pathogen that causes mild to severe diarrheal illnesses and has major public health significance. the aim of this study was to investigate the effects of antimicrobial activity of the gold nanoparticles on vibrio cholera. gold and silver nanoparticles are chemically synthesized. standard strain of vibrio cholerawas cultured in a nutrient broth. minimum inhibitory conc...

Vibrio species are the major seafood-borne bacteria that are frequently associated with the consumption of contaminated sea food. A total of 113 samples including 58 fish and 55 shrimps were studied for the possible contamination with Vibrio species. A biochemical protocol was applied for the identification of the Vibrio isolates and Polymerase Chain Reaction (PCR) was carried out to confirm th...

conclusions our findings suggested a worrying increase in resistance of v. cholerae strains to commonly used antibiotics. differences in patterns of resistance between afghan and iranian patients' samples were observed, which further emphasize a need for constant observation. results a st revealed high levels of resistance to sulfamethoxazole-trimethoprim (89.6%), tetracycline (60.4%), ampicill...