A click reaction (copper-catalyzed azide-alkyne cycloaddition) catalyzed by "naked" copper ions (without ligands) generated in situ from a copper substrate by laser ablation in a high-voltage assisted laser desorption/ionization (HALDI) ion source is demonstrated.

in this article, preparation of polypyrrole and its nanocomposites as adsorbents werediscussed and the capability of separation of copper ions from aqueous solution were studied.polypyrrole was prepared by chemical oxidative polymerization method of pyrrole usingfecl3 as an oxidant. the removal of cu (ii) was investigated using ppy, ppy/tio2 andppy/tio2/dhsna nanocomposites. the products were i...

Background and aims. This study aimed to investigate release of nickel ion from three types of nickel-titanium-based wires in the as-received state and after immersion in a simulated oral environment. Materials and methods. Forty specimens from each of the single-strand NiTi (Rematitan "Lite"), multi-strand NiTi (SPEED Supercable) and Copper NiTi (Damon Copper NiTi) were selected. Twenty specim...

The capacity of metal-dependent fungal and bacterial polysaccharide oxygenases, termed GH61 and CBM33, respectively, to potentiate the enzymatic degradation of cellulose opens new possibilities for the conversion of recalcitrant biomass to biofuels. GH61s have already been shown to be unique metalloenzymes containing an active site with a mononuclear copper ion coordinated by two histidines, on...

Copper ions are essential at a proper level yet toxic when present in excess. To maintain a proper intracellular level, cells must be able to sense the changes in copper ion concentrations. The yeast transcription factor Mac1p plays a critical role in the transcriptional regulation of CTR1 and CTR3, both encoding high affinity copper ion transporters. Here we report that the Mac1p binding of th...

Surfaces made of copper or its alloys have strong antimicrobial properties against a wide variety of microorganisms. However, the molecular mode of action responsible for the antimicrobial efficacy of metallic copper is not known. Here, we show that dry copper surfaces inactivate Candida albicans and Saccharomyces cerevisiae within minutes in a process called contact-mediated killing. Cellular ...

Native electrospray ionization (ESI) mass spectrometry (MS) is often used to monitor noncovalent complex formation between peptides and ligands. The relatively low throughput of this technique, however, is not compatible with extensive screening. Laser ablation electrospray ionization (LAESI) MS combined with ion mobility separation (IMS) can analyze complex formation and provide conformation i...

This journal is a The Royal Society of Separation of metalloproteins using a novel metal ion contaminant sweeping technique and detection of protein-bound copper by a metal ion probe in polyacrylamide gel electrophoresis: distribution of copper in human serum† Shingo Saito,* Mitsuyoshi Kawashima, Hiroki Ohshima, Kazuki Enomoto, Makoto Sato, Hajime Yoshimura, Keitaro Yoshimoto, Mizuo Maeda and M...

Chelation of Cu(I) ions to an immobilized hydrophilic tris(triazolylmethyl)amine chelator on a solid support allowed synthesis of RNA oligonucleotide conjugates from completely deprotected alkyne-oligonucleotides. No oligonucleotide strand degradation or metal ion contamination was observed. Furthermore, use of the immobilized copper(I) ion overcame regioselectivity issues associated with strai...

A disposable copper (II) ion biosensor based on self-assembly of L-cysteine on gold nanoparticle-modified screen-printed carbon electrode was fabricated. The electrode was modified by attaching gold nanoparticles onto the surface of screen-printed carbon electrode through seed mediated growth method followed by self-assembly of L-cysteine. As demonstrated by differential pulse voltammetry, the ...