Background: Patients with fever of unknown origin (FUO) are elusive and challenging clinical cases. Timely detection and identification of blood borne pathogen is one of the most important functions of microbiology laboratory. Objectives: To evaluate the efficacy of BACTEC 9050 in comparison to conventional blood culture for detection of bacterial isolates in clinically suspected cases of fever...

In a comparative fungal blood culture study, a lysis-centrifugation system (Isolator; Du Pont Co., Wilmington, Del.) detected 89% of all episodes of fungemia; the lysis-centrifugation system detected fungemia exclusively or significantly earlier than did a biphasic brain heart infusion bottle system 83% of the time. The lysis-centriguation system was particularly useful in the early detection o...

BACKGROUND Early laboratory detection of Mycobacterium tuberculosis is crucial for controlling tuberculosis. We developed a hydrogel mycobacterial culture method that retains the advantages of both solid and liquid methods in terms of speed, cost, and efficiency. METHODS Mycobacterium bovis bacillus Calmette-Guérin (BCG) suspensions and 200 acid-fast bacilli (AFB)-positive clinical specimens ...

Maintenance of stock cultures of yeast is essential in all mycology laboratories for quality control. Among several methods proposed for maintaining yeast culture collections, periodic subculture on agar slopes is most commonly adopted. In an attempt to reduce cost of preservation of yeast cultures, we evaluated a simple modification of agar slope stock culture technique where 20 strains of yea...

A modified slide culture method which combines advantages found in several slide culture methods is described. A block of inoculated nutrient agar sandwiched between two sterile cover glasses is placed in a plastic petri dish containing water agar. After adequate growth has occurred, the slide culture is disassembled and mounted in a conventional manner.

Bacterial contamination in dental unit waterlines (DUWLs) was evaluated by molecular techniques in addition to the conventional culture method. Water samples (n=8) from DUWLs were investigated for heterotrophic bacteria by culture method using R2A agar. The selected bacterial antibiotic-resistance genes and Legionella species-specific 16SrDNA were identified by PCR. The profiles of bacterial co...

adenoviruses (advs) types 40 and 41 are the causative agents of diarrhea in children. hence, rapid sensitive and specific detection of these viruses are of clinical importance. the customary methods such as propagation of virus in cell culture suffer from limitations. detection of immobilized amplified products in a one phase system (diapops) method has the potential to overcome these problems....

BACKGROUND/PURPOSE A rapid diagnostic method for methicillin-resistant Staphylococcus aureus (MRSA) has been implemented for surveillance of the at-risk population, but its performance in those without traditional risk factors is not clear. The objective of this study was to evaluate MRSA colonization status by comparing the performance of the BD GeneOhm MRSA polymerase chain reaction (PCR) ass...

BACKGROUND Differences in the susceptibility of Candida species to antifungal drugs make identification to the species level important for clinical management of candidemia. Molecular tests are not yet standardized or available in most clinical laboratories, although such tests can reduce the time required for species identification, as compared to the conventional culture-based methods. To dec...