We attempted to determine whether T cells express any VH gene segments. cDNA libraries were constructed from one suppressor and two helper T cell hybridomas. Both the library construction and screening were designed to maximize detection of a wide range of VH gene segments. One screening method should detect about half of the sequenced VH genes, while the second should detect most of these gene...

Speed limits were increased in Utah and other States after repeal of the national maximum speed limit law (NMSL) in 1995. This study analyzed effects of the increased speed limit on Utah highways on crash rates, fatality crash rates, and injury crash rates. Annual (1992-1999) rates of crashes, fatality crashes, and injury crashes for the following highway categories were calculated: urban Inter...

The registration of as-built and as-planned building models is a pre-requisite in automated construction progress monitoring. Due to the numerous challenges associated with process, it still performed manually. This research study proposes an method that aligns point cloud its model using planar features. proposed extracts processes all plane segments from both models, then—for models—groups pa...

This paper extends the RRT* algorithm, a recentlydeveloped but widely-used sampling-based optimal motion planner, in order to effectively handle nonlinear kinodynamic constraints. Nonlinearity in kinodynamic differential constraints often leads to difficulties in choosing appropriate distance metric and in computing optimized trajectory segments in tree construction. To tackle these two difficu...

Precise DNA manipulation is critical for molecular biotechnology. Restriction enzyme-based approaches are limited by their requirement of specific enzyme sites. Restriction-free cloning has greatly improved the flexibility and speed of precise DNA assembly. Most of these approaches focus on DNA assembly rather than gene removal. Here we present a polymerase chain reaction (PCR)-based cloning me...

Radiotracer experiments are carried out in order to determine the mean residence time (MRT) as well as percentage of dead zone, V dead (%), in an integrated mixer consisting of Rushton and pitched blade turbine (PBT). Conventionally, optimization was performed by varying one parameter and others were held constant (OFAT) which lead to enormous number of experiments. Thus, in this study, a 4-fac...

Herein, we present a simple linear motor, built from DNA and a restriction enzyme, which moves a DNA cargo in discrete steps along a DNA track. Movement is powered by a nicking enzyme that cuts the track. Damage to the track in the wake of the cargo imposes directionality. The specificity of base-pairing, the rigidity of short segments of the double helix, and the flexibility of singlestranded ...