Rice (Oryza sativa var. Nipponbare) was transformed with an artificial avidin gene. The features of this construct are as follows: (1) a signal peptide sequence derived from barley alpha amylase was added at the N-terminal region, (2) codon usage of the gene was optimized for rice, and (3) the gene was driven by rice glutelin GluB-1, an endosperm-specific promoter. Avidin was produced in the gr...

Laboratory data show that populations of flour beetles (1ribolium), when grown in a periodically fluctuating volume of flour, can exhibit significant increases in numbers above those attained when grown in a constant volume (of the same average). To analyze and explain this phenomenon a discrete stagestructured model of 1ribolium dynamics with periodic environmental forcing is introduced and st...

The influence of four cereals namely, flours of wheat (Triticum aestivum L.), millet (Pennisetum glaucum L.), sorghum (Sorghum bicolor (L.)Moench) and maize (Zea mays L.)on the growth and development of T. castaneum was investigated at ambient tropical laboratory conditions of 30±3 ̊C and relative humidity of 75±5%. The antinutrients, mineral profile and proximate compositions of the four flour ...

Red flour beetles, Tribolium castaneum (Herbst), and confused flour beetles, Tribolium confusum (DuVal), were exposed for 8-72 h to diatomaceous earth (Protect-It) at 22, 27, and 32 degrees C and 40, 57, and 75% RH (9 combinations). Insects were exposed to the diatomaceous earth at 0.5 mg/cm2 on filter paper inside plastic petri dishes. After exposure, beetles were held for 1 wk without food at...

Long-term efficacy of the protein-enriched flour of pea (Pisum sativum L. var. Bonneville) in its toxicity, progeny reduction and organoleptic properties was evaluated by combining it with wheat flour and testing the admixture against the red flour beetle, Tribolium castaneum (Herbst). The toxicity and progeny-reducing effects of the wheat flour treated with protein-enriched pea flour were stab...

Hermetic storage is used to protect grain against insect pests, but its utility is not limited to whole grains. We evaluated hermetically-sealed, polyethylene terephthalate (PET) bottles for preserving wheat and maize flour against red flour beetle (RFB, Tribolium castaneum, Herbst) population growth. Flours infested with RFB and kept in sealed PET bottles experienced much less weight loss over...

Late-instar larvae, pupae, and adults of Tribolium castaneum (Herbst), the red flour beetle, or Tribolium confusum (DuVal), the confused flour beetle, were exposed for variable durations at 36–54 1C. Beetles were placed in laboratory ovens set at a baseline of 27 1C, the temperature was increased by 0.1 1C per minute until the target temperature was achieved, and beetles were then held for spec...

Synthesis of bicyclic [1] and tricyclic [2] terpenoid lactones and the preliminary results of biological tests for their feeding deterrent activity [3] previously were presented. The tests, which were carried out on three storage pest insects: the grain weevil (Sitophilus granarius L.), the confused flour beetle (Tribolium confusum Duv.) and the khapra beetle (Trogoderma granarium Ev.), showed ...

The relationship between number of holes in a grain probe trap body and capture of stored-grain pests was determined in laboratory tests using adults of the rice weevil, Sitophilus oryzae (L.), the sawtoothed grain beetle, Oryzaephilus surinamensis (L.), and the red flour beetle, Tribolium castaneum (Herbst). Polyvinylchloride (PVC) probe bodies were attached to electronic sensor heads, and ins...