gene. in phage, may be defined as the hereditary information determining A the primary structure of a polypeptide chain. In the absence of information concerning mutational alterations of specific proteins, the operational definition of a gene is usually derived from complementation tests. Cells containing two functioning genomes with different recessive mutations in the trans configuration are...

In mutagenesis screens for recessive female sterile mutations on the second chromosome of Drosophila melanogaster 529 chromosomes were isolated which allow the homozygous females to survive, but cause them to be sterile. In 136 of these lines, mutant females produce morphologically normal eggs which cannot support normal embryonic development. These "maternal-effect" mutations fall into 67 comp...

Rubella virus (RUB) replicons with an in-frame deletion of 507 nucleotides between two NotI sites in the P150 nonstructural protein (DeltaNotI) do not replicate (as detected by expression of a reporter gene encoded by the replicon) but can be amplified by wild-type helper virus (Tzeng et al., Virology 289:63-73, 2001). Surprisingly, virus with DeltaNotI was viable, and it was hypothesized that ...

To better understand the health implications of personal genomes, we now face a largely unmet challenge to identify functional variants within disease-associated genes. Functional variants can be identified by trans-species complementation, e.g., by failure to rescue a yeast strain bearing a mutation in an orthologous human gene. Although orthologous complementation assays are powerful predicto...

The paper delineates the problem related to difference and complementation in Multiset Theory. It is shown that none of the existing approaches succeeds in resolving the attendant difficulties without assuming some contrived stipulations. Mathematics Subject Classifications: 03E15, 03E20, 03E30

The complementation problem for nondeterministic word automata has numerous applications in formal verification. In particular, the language-containment problem, to which many verification problems is reduced, involves complementation. For automata on finite words, which correspond to safety properties, complementation involves determinization. The 2 blow-up that is caused by the subset constru...

Complementation of Büchi automata has been studied for over five decades since the formalism was introduced in 1960. Known complementation constructions can be classified into Ramsey-based, determinization-based, rank-based, and slice-based approaches. Regarding the performance of these approaches, there have been several complexity analyses but very few experimental results. What especially la...

This thesis argues that clausal arguments of mental-state predicates divide into two main types: those that express the content, or "subject matter" of the mental state, and those that express the cause of the mental state. My central theoretical claim is that this dichotomy corresponds to a difference in syntactic structure. I propose that these different structures derive from an operation of...

general theory of interallelic complementation has been proposed by CRICK A and ORGEL (1964), and this has been used by ISHIKAWA (1965) to interpret the relationship between the complementation map and the recombination map of the ad-8 locus in Neurospora crassa and has also been used to interpret a more complex complementation map at the leu-2 locus in Neurospora crassa (GROSS 1962; GILLIE 196...