Natural products have coming from various source materials including terrestrial plants, terrestrial microorganisms, marine organisms, terrestrial vertebrates and invertebrates have importance as they provide an amazing source of new drugs as well as new drug leads and new chemical entities for further drug development (McCurdy & Scully, 2005; Chin et al., 2006). Morphine, vincristine, codeine,...

Several column chromatographic methods are available for the separation of aflatoxins (l-6). Most of these procedures are elaborate and involve several steps of purification. A rapid and simple method of obtaining pure aflatoxins was reported by Steyn (5); however, with this method, we were not able to obtain pure crystalline aflatoxins B, and G1 for our experimental studies on animals. When pu...

The optimum parameters of extraction for glycoprotein from seahorse were examined and determined by Box-Behnken combined with ultrasonic extraction technology. Column chromatography of glycoprotein was used for further purification. The optimal extraction conditions of seahorse glycoprotein were extracting time 4.3 h, salt concentration 0.08 mol/L, extracting temperature 73°C, raw material, and...

Injectors/Autosamplers 2815 Temperature Effects In LC 2815 Sample Preparation/Derivatization 2816 Microchip Technology in LC 2816 Automation 2817 Other Articles of Interest 2817 Columns 2817 Reviews 2817 Columns Materials, Effects, and Packing 2817 Supports 2817 Stationary Phases 2818 Ion-Exchange Phases 2819 Monolithic Columns 2819 Molecular Imprinted Polymer (MIP) Phases 2819 Tunable Stationa...

Mercurated dextran (polysaccharide) was found to fractionate mononucleotides according to their affinity for organomercurial Hg(+). The nucleotides are released from the chromatographic column in the sequence CMP, AMP, GMP, and TMP when borate buffer of increasing pH is used as an eluent.

An automated solid phase extraction (SPE) protocol to determine carbamazepine in human serum has been developed and validated using a simple, rabid sensitive liquid chromatography-based bio-analytical method. Extraction of was carried out an on-line SPE tool short protein-coated (PC) ODS silica pre-column (PC-ODS-pre-column) phosphate buffer saline (PBS) with pH 7.4 as solvent. There are two di...