BACKGROUND The aim of this study was to investigate the potential function of miR-126 in neural stem cells (NSCs). MATERIAL AND METHODS Expression level of miR-126 was detected by quantitative real-time PCR (qRT-PCR). MiR-126 overexpression was established by transfecting miR-126 mimics into human NSC lines (HB1.F3 and HB1.A4 cells). Its effects on cell proliferation were studied using cell-c...

Objective: This work aimed to enhance colony polymerase chain reaction (PCR) for the 16S rRNA of several Escherichia coli strains. Methods: The isolation E. is done from gut chicken and soil. Then, we optimized condition PCR amplification 16s ribosomal RNA. We successfully designed primer 3 RNA made dilution solution with grade water that 1:10. Moreover, finally, a 20 μL contains master mix our...

Background: As condition and component of culture determine fate map of spermatogonial stem cells (SSCs), the aim of this study was to evaluate of growth factors GDNF, LIF and RA on proliferation and differentiation of SSC. Materials and Methods: SSCs were cultured in two groups: The first group GDNF and LIF and the second group RA. The number of clumps and colony formation was monitored dur...

This study aims at molecular identification of Salmonella Infantis isolated from backyard chickens and the detection of their antibiotic resistance genes. A total of 46 Salmonella-suspected samples isolated from backyard chickens of northern Iran were collected. Serotyping was done by the traditional method and then confirmed by PCR. Antimicrobial susceptibility of the isolates against 13 antim...