In previous studies we found that a mild thermal burn of the vitamin A-deficient rat cornea caused collagenase release into the medium of corneas placed in culture media 72 hr after applying the burn. Collagenase was released only on day 1 of culture and was not released from identically burned corneas of control rats. We now demonstrate that in deficient corneas, this collagenase release on da...

The aim of the study was to evaluate the relationship between the time from femoral neck fracture and the content of transforming growth factor-beta1 (TGF-beta1), interleukin-1beta (IL-1beta) and collagenase activity in bone samples of the femoral neck. The material consisted of 42 cancellous bone samples from the femoral neck collected from patients after the femoral neck fracture during hip r...

There is ample evidence to indicate an important role for specific collagenases in the turnover of connectivetissue collagens both in normal and pathological conditions (Harris & Krane, 1974a,b,c). It has been the aim of many investigators to define the stimuli controlling the synthesis and secretion of collagenase, but it is clear that the extracellular control of the activity of collagenase i...

Asthma airway remodeling is characterized by the thickening of the basement membrane (BM) due to an increase in extracellular matrix (ECM) deposition, which contributes to the irreversibility of airflow obstruction. Interstitial collagens are the primary ECM components to be increased during the fibrotic process. The aim of the present study was to examine the interstitial collagen turnover dur...

BACKGROUND In phytotherapy, the therapeutic potential is based on the combined action of different herbal drugs. Our objective was to evaluate the antioxidant, anti-collagenase (in vitro), and anti-wrinkle (in vivo) effect of combined formulation containing Ginkgo biloba, Punica granatum, Ficus carica, and Morus alba fruits extract. METHODS Antioxidant evaluation was based on the scavenging a...

1. Explants of rabbit skin and synovium in tissue culture secreted a specific collagenase into their culture media. Primary cultures of fibroblast-like cells, which were obtained from these tissues and maintained in culture for up to 14 subculture passages, also secreted high activities of a specific collagenase into serum-free culture medium. Secretion of enzyme activity from the cell monolaye...

Fibroblast cultures derived from human basal cell carcinomas demonstrated an increased capacity to synthesize and secrete collagenase. Although the levels of collagenase were up to 8-fold greater than those of normal control cell lines, this phenotypic trait was not permanent and was expressed only for a few passages following primary explanation. The basal cell carcinoma fibroblast collagenase...