Collagenase production by chondrocytes appears to play a major role in the development of osteoarthritis. Although the mechanisms regulating collagenase production by chondrocytes are not known, incubation of bovine chondrocytes in serum markedly decreases collagenase production. Since serum has been demonstrated to increase levels of phosphotyrosine (P-Tyr) in several cell types, we determined...

OBJECTIVE This study focuses on the growth-promoting and migration-enhancing role that Clostridial collagenase plays in vitro and in vivo. METHODS For in vitro studies, biosynthesized extracellular matrices were treated with purified Clostridial collagenase, nonspecific proteases, or buffer controls. Keratinocytes were subsequently plated upon these matrices in the presence or absence of Clos...

We prepared a cell sheet by using collagenase treatment to digest salmon atelocollagen fibrillar gel (SAC gel) on which human periodontal ligament (HPDL) cells had been cultured. The SAC gel was found to be digested completely within 2 h at a concentration of 50 U of collagenase per mg of collagen. The SAC gel on which HPDL cells were cultured for 10 d was treated with collagenase, resulting in...

There is ample evidence to indicate an important role for specific collagenases in the turnover of connectivetissue collagens both in normal and pathological conditions (Harris & Krane, 1974a,b,c). It has been the aim of many investigators to define the stimuli controlling the synthesis and secretion of collagenase, but it is clear that the extracellular control of the activity of collagenase i...

Zoanthamine-type alkaloids display a wide spectrum of biological effects. This study aimed to examine the inhibitory effects of norzoanthamine and its ten homologues of zoanthamine class on human fibroblast collagenase by modeling a three-dimensional structure of the ligands at collagenase using energy minimization, docking, molecular dynamics simulation and MM-PB/GBSA binding free energy calcu...

Using the method of dynamic light scattering, it was found that rate collagen cleavage under influence bacterial collagenase decreases by almost 4 times when protein molecules are doped with Cr 3+ ions, and enzyme activator Zn 2+ ions is added, increases 1.3 times. Based on experimental data, reaction constants k 1 were calculated. It tanned a chromium salt, becomes more resistant to degradatio...

Collagenase enzyme activity of 38 Pseudomonas aeruginosa strains and 38 strains of Escherichia coli from various pathological sources was measured by a simple method. This method uses plates with collagen gel. The rate of gel lysis is proportional to the collagenase concentration. The method is simple and requires no special materials or equipment. From the 38 P. aeruginosa strains, 34 were col...

The level of phosphodiesterase (PDE) activity is lower in collagenase-isolated human fat cells than in adipose tissue fragments. The inhibition is not species-specific since collagenase also inhibits PDE in rat adipose tissue and bovine heart. In subcellular fractions from isolated fat cells, the PDE activities were lowest in the plasma membrane-enriched fractions and highest in the cytosolic f...

Activated ras oncogene transfection into suitable recipient cells has been shown to induce the metastatic phenotype (Thorgeirsson, et al., Mol. Cell. Biol., 5: 259-262, 1985). We have used this model system to study the correlation of basement membrane collagenolysis with metastatic propensity. The c-Ha-ras oncogene alone, or combined with v-myc, transfected into early passage rat embryo fibrob...

We have previously shown that during wound healing migrating keratinocytes, which are in contact with the dermal matrix, express interstitial collagenase, whereas basal epidermal cells, which reside on an intact basement membrane, do not. Duplicating this in vivo pattern, collagenase production was induced in primary human keratinocytes grown on native type I collagen, but only background level...