Expression of type I and type IV collagen genes was studied in normal endothelial cells and in modulated endothelial cells (formerly designated as fibroblastic corneal endothelial cells: FCEC). There appear to be distinct collagen phenotypes in these cells; normal cells produce type IV collagen as a major type, while the modulated cells produce predominantly type I collagen. The absence of type...

In the integrin family, the collagen receptors form a structurally and functionally distinct subgroup. Two members of this subgroup, alpha(1)beta(1) and alpha(2)beta(1) integrins, are known to bind to monomeric form of type I collagen. However, in tissues type I collagen monomers are organized into large fibrils immediately after they are released from cells. Here, we studied collagen fibril re...

Journal of Orthopaedic Research 23 (2005) 142-149 Journal of Orthopaedic Research www.elsevier.com/locate/orthres Spatial organization of types I and II collagen in the canine meniscus Helen E. Kambic, Cahir A. McDevitt* Department of Biomedical Engineering and Orthopaedic Research Center, Lerner Research Institute, The Cleveland Clinic Foundation, ND-20, 9500 Euclid Avenue, Cleveland, OH 44195...

Novel type I collagen hybrid fibrils were fabricated by neutralizing a mixture of type I fish scale collagen solution and type I porcine collagen solution with a phosphate buffer saline at 28 °C. Their structure was discussed in terms of the volume ratio of fish/porcine collagen solution. Scanning electron and atomic force micrographs showed that the diameter of collagen fibrils derived from th...

To investigate the relationship of collagen types I and V within corneal fibrils, and the collagenolytic mechanisms potentially involved in corneal development and remodeling, we have incubated cryostat sections of avian corneas with collagenases that specifically degrade collagen types I or V to digest selectively the collagen in situ. These preparations were then analyzed by immunofluorescenc...

introduction & objective: the brain choroids plexus (bcp) plays an important role in the cerebrospinal fluid (csf) production, but its characterization is still incomplete. collagen type iv, is one of the most important proteins of basement membrane (bm) and extracellular matrix (ecm) of bcp. in the present study we investigated the differential period of type iv collagen in basement membrane o...

background: nicotine can pass through placental blood barrier and accumulate in the developing organs of fetus. also, entering the breast milk, nicotine can have an effect on the neonates. investigations have showed that collagen iv is one of the most important micro vessels basement membrane components. objective:  in this study, the effect of maternal nicotine exposure in pre and postnatal pe...