Objective— Use of equine autologous keratinocyte on collagen membrane grafts (KCMG) for treatment of wounds in the distal aspect of the horse limb. Design— Experimental study Animals— Four horses. Procedure—Keratinocytes have been separated by enzyme digestion from lib skin sample and proliferated in vitro. Full thickness excision wounds (6.25 cm2) were created on the mid-lateral of both met...

BACKGROUND Hypertrophic cardiomyopathy (HCM) is a primary cardiac disease with a diverse clinical spectrum, in which many of the abnormal structural and pathophysiologic features are consequences of inappropriate left ventricular hypertrophy. METHODS We analyzed the amount, distribution and structure of the cardiac collagen network in transmural sections of the ventricular septum (thickness 1...

OBJECTIVE The aim of this study was to evaluate the regenerative potential of cell-laden and cell-free collagen matrices in comparison to microfracture treatment applied to full-thickness chondral defects in an ovine model. METHODS Animals (n = 30) were randomized into 5 treatment groups, and 7-mm full-cartilage-thickness defects were set at the trochlea and medial condyle of both knee joints...

Recent observations suggest that cells on fibrous extracellular matrix materials sense mechanical signals over much larger distances than they do on linearly elastic synthetic materials. In this work, we systematically investigate the distance fibroblasts can sense a rigid boundary through fibrous gels by quantifying the spread areas of human lung fibroblasts and 3T3 fibroblasts cultured on slo...

We investigate the system size-dependent rheological response of branched type I collagen gels. When subjected to a shear strain, the highly interconnected mesh dynamically reorients, resulting in overall stiffening of the network. When a continuous shear strain is applied to a collagen network, we observe that the local apparent modulus, in the strain-stiffening regime, is strongly dependent o...

Purpose: To compare the post-extraction dimensional changes following extraction alone or extraction plus ridge preservation with an intrasocket mineralized freeze-dried bone allograft and a collagen membrane. 2 purpose is to determine whether there are significant differences between 2 procedures histologically.3 one is that a 4-monts and 6month implant placement time was evaluated to determin...

Experimental animal models are frequently used to study articular cartilage, but the relevance to man remains problematic. In this study animal models were compared by examination of the collagen fibre arrangement in the medial tibial plateau of human, cow, pig, dog, sheep, rabbit and rat specimens. 24 cartilage samples from each species were prepared and maximum cartilage thickness in the cent...

Biopolymers such as carrageenan or collagen can be used carriers for loading a drug to enhance drug’s bioavailability. In this work, allopurinol was loaded on carrageenan/collagen blend and the carrageenan/collagen/allopurinol (CCA) biocomposite films were prepared using ionic gelation method combined with 3D printing gel ink material. The advantages of are ease in shaping design controlling th...

OBJECTIVE The objective of this study was to assess the feasibility of transplanting embryonic chondrogenic cells within a collagen-fibrin substrate for the reconstitution of full-thickness cartilage defects in chicken knee joints. METHODS Full-thickness cartilage defects were created mechanically on the weight-bearing surface of the tibial condyle in 45 adult chickens and subsequently filled...

Collagen-hydroxyapatite interfaces compose an important building block of bone structures. While it is known that the nanoscale structure of this elementary building block can affect the mechanical properties of bone, a systematic understanding of the effect of the geometry on the mechanical properties of this interface between protein and mineral is lacking. Here we study the effect of geometr...