MicroRNA (miRNA) detection by reverse transcription (RT) quantitative real-time PCR (RT-qPCR) is the most popular method currently used to measure miRNA expression. Although the majority of miRNA families are constituted of several 3'-end length variants ("isomiRs"), little attention has been paid to their differential detection by RT-qPCR. However, recent evidence indicates that 3'-end miRNA i...

one strain each and by trimethoprim for three strains were reduced (by 2, 6 and 6–7 mm, respectively) after incubation in a CO 2-containing atmosphere compared with those obtained following incubation in the aerobic atmosphere. None of the seven isolates was CO2dependent and in all cases, the susceptibility categories for the relevant antibiotics were downgraded from susceptible in the aerobic ...

to investigate the influence of hypoxia during the early stage of incubation on embryonic developmentand hatching events, and consequently on incidence of ascites in broiler chickens, one thousand fertile eggswere incubated in two commercial incubators. half the eggs were incubated in a low altitude incubator untilhatched. the second half were incubated in a high altitude incubator until day 10...

knowledge of virus and replication kinetic is one of the most important issues in the vaccine production. the present study aimed to evaluate the best harvesting time of h9n2 avian influenza virus (aiv) vaccine strain inoculated in specific pathogen free (spf) embryonated chicken eggs (ece)s. for this purpose, 10-5 dilution of aiv (a/chicken/iran/99/h9n2) was inoculated into 336, 11-day old ece...

Co-culture of bone forming cells and endothelial cells to induce pre-vascularization is one of the strategies used to solve the insufficient vascularization problem in bone tissue engineering attempts. In the study, primary cells isolated from 2 different tissues of the same animal, rat bone marrow stem cells (RBMSCs) and rat aortic endothelial cells (RAECs) were co-cultured to study the effect...

̇ ̇ Ž . Ž . We measured oxygen consumption V and carbon dioxide emission V rates, air-cell gas partial pressures of O CO 2 2 Ž . Ž . Ž oxygen P O and CO P CO , eggshell water vapour conductance and energy content of the ostrich Struthio A 2 2 A 2 ̇ . Ž . camelus egg, ‘true hatchling’ and residual yolk, and calculated RQ and total oxygen consumption V for ostrich O tot 2 ̇ eggs incubated at 36.5 C a...

Hamster intrinsic factor (IF) preparations markedly enhanced the uptake of (57)cobalt-labeled cyanocobalamin (B(12)-(57)Co) by brush borders and microvillous membranes isolated from villous absorptive cells obtained from the distal but not the proximal half of hamster intestine. A similar effect was observed with rat and rabbit IF preparations, but IF preparations obtained from man, dog, and ho...