DNA size markers are widely used to estimate the size of DNA samples on agarose or polyacrylamide gelelectrophoresis (PAGE). DNA markers can be prepared by mixing PCR products with definite sizes.Alternatively, they are prepared by restriction enzyme digestion of the genomic DNA of bacteriophages ornatural and synthetic DNA plasmids. The present study describes engineering of ...

Information about taste signaling molecules is still insufficient to understand the mechanisms of taste signal transductions, while novel taste receptors have been found recently. There are two strategies to discover novel taste signaling related genes; one strategy is homology based cloning and another strategy is global gene screening. Homology based cloning has been mainly used to find out t...

6.1 Overview In Chapter 5 a new universal cloning strategy for the production of specifically designed surface-active PAA’s was introduced. Here, we apply this strategy to the production of a unique surface-active PAA designed to form brush layers on aluminum oxide surfaces. This chapter discusses the procedure taken in assembling the gene encoding for a PAA diblock consisting of a long zwitter...

The design and generation of DNA constructs is among the necessary but generally tedious tasks for molecular biologists and, typically, the cloning strategy is restricted by available restriction sites. However, increasingly sophisticated experiments require increasingly complex DNA constructs, with an intricacy that exceeds what is achievable using standard cloning procedures. Many transgenes ...

To facilitate searching for genes encoding cell membrane proteins, we developed a method for isolating cDNAs that contain sequences for hydrophobic transmembrane runs. This cloning strategy, termed the "transmembrane (TM) trap method," utilizes a vector that directs the cell surface expression of mouse CD4 fusion protein when an insert encoding hydrophobic transmembrane sequences is cloned in-f...