Rat embryonic stem cells (ESCs) offer the potential for sophisticated genome engineering in this valuable biomedical model species. However, germline transmission has been rare following conventional homologous recombination and clonal selection. Here, we used the CRISPR/Cas9 system to target genomic mutations and insertions. We first evaluated utility for directed mutagenesis and recovered clo...

The throughput of DNA reading (i.e., sequencing) has dramatically increased recently owing to the incorporation of in vitro clonal amplification. The throughput of DNA writing (i.e., synthesis) is trailing behind, with cloning and sequencing constituting the main bottleneck. To overcome this bottleneck, an in vitro alternative for in vivo DNA cloning needs to be integrated into DNA synthesis me...

Solid tumors can be highly aneuploid and many display high rates of chromosome missegregation in a phenomenon called chromosomal instability (CIN). In principle, aneuploidy is the consequence of CIN, but the relationship between CIN and aneuploidy has not been clearly defined. In this study, we use live cell imaging and clonal cell analyses to evaluate the fidelity of chromosome segregation in ...

Everyday listening involves a complex interplay between the ear, which transduces sound energy into neural responses, and the brain, which makes sense of these inputs. Historically, research on the ear tended to ignore the fact that what we can perceive in sound depends on what task the brain is engaged by, while research on cortical processing of sound ignored the complexity and sophistication...

Well prepared traffic scenarios contribute greatly to the success of controller-in-the-loop simulations. This paper describes each stage in the design process of realistic scenarios based on real-world traffic, to be used in the Airspace Operations Laboratory for simulations within the Air Traffic Management Technology Demonstration 1 effort. The steps from the initial analysis of real-world tr...

Gestational diabetes(GDM) is increasing exponentially. To address this, we contend that a pervasive mobile solution that can facilitate self-care is a prudent strategy. To demonstrate proof of concept, user fidelity, usability and acceptability of such a solution a small pilot study of 10 patients was designed. A quasi-experimental approach employing a randomized control trial with a two period...

In this paper the reconstruction of damaged piecewice constant color images is studied using a RGB total variation based model for colorization/inpainting. In particular, it is shown that when color is known in a uniformly distributed region, then reconstruction is possible with maximal fidelity.

conclusions our results illustrated the presence of clonal groups of highly gentamicin-resistant strains of mrsa in hospitals in tehran. the php typing method provided useful information for both clonal dissemination and determining the epidemiological links of the clonal groups of the mrsa strains. materials and methods a total of 301 s. aureus isolates were collected during january to novembe...