In this study, search for new mosquitocidal bacterium was attempted from the gut content of fresh water fish Clarias batrachus (walking cat fish) which resulted in isolation a highly potential bacterium. The isolate identified as Bacillus cereus VCRC 641 by species specific molecular markers ilvD, pur and pycA gene sequence analysis. Laboratory mosquito larval toxicity assay (bioassay) against ...

Bacillus cereus VCRC 641 was found to be an alternate larvicidal bacterial agent which newly isolated from fresh water fish, Clarias batrachus (walking cat fish). The protein responsible for mosquito toxic effect separated and purified by Sephacryl S-200 column chromatography NATIVE PAGE. It observed that a unique with molecular weight of 70kDa the factor killing effect. Further analysis liquid...

Blood is collected from experimental animals for a wide range of scientific purposes including; hematology, clinical biochemistry parameters, immunology, studies in bacteriology, parasitology and investigations in reproductive performance and health. The number of methods employed to collect blood from fish include; the puncture of caudal vein, dorsal aorta or cardiac vessels and the severance ...