Extracts of normal brains obtained at autopsy and cerebrospinal fluid (CSF) from patients with global brain ischemia were analyzed for creatine kinase (CK; EC 2.7.3.2) isoenzymes. We used both qualitative and quantitative assays (electrophoresis and immunoinhibition). Brain extracts contained CK-BB isoenzyme and mitochondrial CK. In 54 CSF samples free of blood contamination and with total acti...

Intermediate filaments are involved in cell migration and intracellular signal transduction pathways. In a variety of organs, the expression of distinct intermediary filaments are further associated with distinct steps of malignant transformation. In this study, we seeked to define the cytokeratin (Ck) expression pattern in oral leukoplakia and oral squamous cell carcinoma (OSCC). One hundred a...

B A C K F I R E ? In late June, Hong Kong media reported China’s PLA-AF (People’s Liberation Army – Air Force) would seek to become a ‘strategic air force’ with the acquisition of the Russian Backfire cited as a core tenet of this fundamental policy change. To best appreciate the potential strategic impact of the Backfire upon the Asia Pacific region it is worth exploring the capabilities of th...

This note describes possible world semantics for a constructive modal logic CK. The system CK is weaker than other constructive modal logics K as it does not satisfy distribution of possibility over disjunctions, neither binary (3(A ∨ B) → 3A ∨ 3B) nor nullary (3⊥ → ⊥). Our intuition is that 3⊥ should not constructively imply ⊥, thus we dispense with both forms of distribution. We are intereste...

Certain types ofmacro-CK, type 1, have been reported to cause false increases in CK-MB by co-migration on electrophoresis (1-3). I report two such cases in which the macroCK was an IgA with anti-CK-BB specificity. According to methods proposed by Medeiros et al. (1), immunoprecipitation and subsequent analysis by elecrophoresis was used to separate interfering macre-CK from CK-MB. CK electropho...

Partindo da análise das manifestações de insatisfação usuários do Spotify no Twitter com o resultado Retrospectiva 2019, pretende-se investigar como a atuação dos algoritmos nas plataformas streaming música pode agenciar prática sonora ouvintes, tendo gosto musical um principais marcadores fruição ambiente digital. O artigo promove resgate conceito performance e busca repensá-lo partir dinâmica...

Serum creatine kinase isoenzyme 2 concentrations (CK 2 mass) were measured in marathon runners during training and 1 and 2 days after a race and compared with values from 36 acute myocardial infarction (AMI) patients whose total CK and (or) CK 2 activities were similar to those of runners in the basal state. During training, runners had CK and CK 2 activities 53% and 43% above reference values,...

The isoforms of CK-MB have recently received attention as potential biochemical markers for the early diagnosis of an acute myocardial infarction. A sensitive (analytical sensitivity = 0.2 ng/ml) immunochemical mass assay for the direct measurement of the CK-MB2 isoform has been developed by us. This assay utilizes a specific monoclonal capture antibody directed against the B-subunit of CK-MB a...

The enzyme creatine kinase (CK) [EC 2.7.3.2] is a dimeric molecule consisting of two separate subunits Band M, giving rise to three isoenzymes CK-BB, CK-MB and CK-MM. Most of the CK found in the plasma of normal individuals is the MM isoenzyme derived from skeletal muscle, while the CK-MB fraction originates predominately from cardiac muscle. Normally there is little, if any, CK-BB isoenzyme pr...