OBJECTIVES We examined whether unloading of the left ventricle with a ventricular assist device (LVAD) can result in normalization of the creatine kinase (CK) abnormalities in the failing human heart. BACKGROUND Left ventricular failure is associated with a decrease of myocardial total CK activity and a fetal shift in CK isoform expression that results in an increase in the cytosolic brain ty...

The creatine kinase (CK) isoenzyme system is essential for motility in rooster and sea urchin sperm. In the present study, biochemical characterization as well as immunofluorescence and confocal laser microscopy with highly specific antibodies against various chicken CK isoenzymes revealed that cytosolic brain-type CK isoenzyme (B-CK) is the only CK isoenzyme in rooster seminal plasma, while th...

Let CK∗ denote the C ∗-algebra defined by the direct sum of all Cuntz-Krieger algebras. We introduce a comultiplication ∆φ and a counit ε on CK∗ such that ∆φ is a nondegenerate ∗-homomorphism from CK∗ to CK∗ ⊗ CK∗ and ε is a ∗-homomorphism from CK∗ to C. From this, CK∗ is a counital non-commutative non-cocommutative C ∗bialgebra. Furthermore, C∗-bialgebra automorphisms, a tensor product of repr...

Creatine kinase (CK) provides ATP buffering in skeletal muscle and is expressed as 1) cytosolic myofibrillar CK (M-CK) and 2) sarcomeric mitochondrial CK (ScCKmit) isoforms that differ in their subcellular localization. We compared the isometric contractile and fatigue properties of 1) control CK-sufficient (Ctl), 2) M-CK-deficient (M-CK[-/-]), and 3) combined M-CK/ScCKmit-deficient null mutant...

Aim: The aim of the study was to investigate the effects of in vitro hemolysis on serum creatine kinase and creatine kinase MB isoenzyme activities with creatine kinase MB mass, troponin I and myoglobin measurements. Materials and methods: We prepared serum pools having analyte concentrations at normal and pathological values. Hemolysate was added into serum pools to obtain aliquotes with final...

We evaluated the analytical performance of the immunoprecipitation technique for quantification of creatine kinase (CK; EC 2.7.3.2) isoenzyme MB, focusing on specimens with increased blank activities. The samples we studied had blank values that were equal to or greater than either 15 U/L or the uncorrected CK-MB value. Of 134 specimens selected, 16 and 21 contained macro CK type 1 and CK-BB, r...

Let CK∗ denote the C ∗-algebra defined by the direct sum of all Cuntz-Krieger algebras. We introduce a comultiplication ∆φ and a counit ε on CK∗ such that ∆φ is a nondegenerate ∗-homomorphism from CK∗ to CK∗ ⊗ CK∗ and ε is a ∗-homomorphism from CK∗ to C. From this, CK∗ is a counital non-commutative non-cocommutative C ∗bialgebra. Furthermore, C∗-bialgebra automorphisms, a tensor product of repr...

We analytically and clinically evaluated Abbott's IMx assay for creatine kinase (CK) isoenzyme MB (CK-MB) in serum. Over a 1-year period, the method was more specific but less precise than catalytic isoenzyme measurements by electrophoresis or immunoinhibition. Sera from different individuals without electrophoretic evidence of CK-MB but containing macro CK type 1 (n = 20), mitochondrial CK (n ...