BACKGROUND Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods to express and purify these proteins in a parallel, time and cost effective manner. RESULTS We developed a method for parallel expression and purification of recombinant proteins with a hexahistidine tag (H...

A practically simple top-down process for the exfoliation of graphene (GN) and few-layer graphene (FLG) from graphite is described. We have discovered that a biocompatible amphiphilic pyrene-based hexahistidine peptide is able to exfoliate, functionalize, and dissolve few layer graphene flakes in pure water under exceptionally mild, sustainable and virtually innocuous low intensity cavitation c...

Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an excellent solubility enhancer, MBP is not a particularly good affinity tag for protein purifi...

A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

with regard to the evidences of the rule, what is inferred from the qur’an and the tradition (sunna) about the matter of authority over possessions, is the impermissibility of other’s invasion of human self, which is totally unrelated to the assertion of one’s authority over oneself. the realm and the quality of human’s authority over his own organs are not known to the wise, as the relevant po...

Choosing death, whether as a terminally ill patient refusing further treatment or as a death row inmate refusing further appeals of a death sentence, invariably raises questions of mental state. In answering them, we strive to assess diagnoses objectively and employ competency criteria that balance preservation of autonomous decision-making with more paternalistic goals. Two recent developments...

hakim sanaie lived in last parts of fifth and first of sixth centuries. he used and compiled the mystical, ethical and quranic concepts in his poetry. he influenced attar and molavi. sanaie started to compose the mystical and ethical versified story. he has used some parts of verses, themes, definition, words and composition of quran in his poetry. the present essay deals with introducing sanai...