An amperometric biosensor for the detection of polyphenols in wine has been developed immobilizing the two enzymes Tyrosinase and Laccase on graphite screen printed electrodes modified with ferrocene. Different immobilization procedures have been carried out, the sensor operational parameters have been optimized, determining the best conditions and the analytical method for the analysis of samp...

Abstract The hydrophilic and lipophilic antioxidant activities due to the main bioactive components present in Spanish tomato paste samples were studied, using standardized fluorescent methods. After extraction, phenolic antioxidants (Folin-Ciocalteu method) total activity (TEAC assay) evaluated, examining differences between extracts corresponding different samples. Total fluorescence spectra ...

Limonium brasiliense is a common plant on the southern coast of Brazil. The roots are traditionally used for treatment of premenstrual syndrome, menstrual disturbances and genito-urinary infections. Pharmaceutical preparations obtained from its roots and used for these purposes were marketed in Brazil in the 1980s and 1990s. Currently, the Brazilian Drug Agency (National Health Surveillance Age...

In recent years the concept of the specificity of enzyme action has been subjected to revision. The proteolytic enzymes (1) have been shown to have esterase activity and the concept of a blood esterase as distinct from a blood lipase is no longer tenable (2). The enzymatic hydrolysis of the steroid hormone esters by blood sera (3) had not been suspected until solubility determinations of the es...