The purpose of the present study is to investigate the cytotoxicity/apoptotic effect of 2-chloro-2′-deoxyadenosine, cladribine, (2-CdA) in the human breast cancer cell line, MDA-MB468 (estrogen receptor negative, ER−). MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide] assay, annexin V-Fluorescein/PI and Hoechst 33258 staining were used to detect cytotoxicity and cell apopto...

background: soil structure is mainly composed of sand, silt, clay, and organic materials. organisms can live in the soil. the large number of stray cats in the cities of iran is a major environmental and health problem. toxoplasma oocysts are shed with the feces of cats, so soil is known as a potential source of transmission of toxoplasmosis. the aim of this study was to determine the soil cont...

The following method for staining connective tissue is simple and is believed to be better than any yet proposed for that purpose. It is not absolutely differential because, besides connective-tissue fibrill~e and reticulum, it also stains certain hyaline substances, but these latter usually are so different morphologically that confusion cannot arise. The method is also useful for the study of...