background: flow cytometry (fcm) has been extensively used to study mammalian sperm in the areas of clinical andrology and reproductive toxicology. fcm provides a powerful advantage over microscopy technique in terms of rapid accurate and reproducible technology for the quantification of various cell characteristics including chromatin status. during spermiogenesis histones are replaced by prot...

This study was done to evaluate the effect of sperm source on chromatin integrity and ICSI outcomes. One hundred and thirteen samples containing epididymal aspirates of 57 obstructive azoospermic men and 56-ejaculated semen of normozoospermic men were included in this study. Sperm chromatin status was evaluated by Chromomycin A3 (CMA3), Aniline Blue (AB) and Toluidine Blue (TB). Fertilization r...

Genome-wide mapping of protein-DNA interactions is critical for understanding gene regulation, chromatin remodeling, and other chromatin-resident processes. Formaldehyde crosslinking followed by chromatin immunoprecipitation and high-throughput sequencing (X-ChIP-seq) has been used to gain many valuable insights into genome biology. However, X-ChIP-seq has notable limitations linked to crosslin...

Development and acclimation processes to the environment are associated with large-scale changes in chromatin compaction in Arabidopsis (Arabidopsis thaliana). Here, we studied the effects of light signals on chromatin organization. A decrease in light intensity induces a large-scale reduction in chromatin compaction. This low light response is reversible and shows strong natural genetic variat...

Introduction: Date palm gemmule has been used in traditional medicine to improve the semen quality and treat infertility. Their components remedies those are rich of antioxidant and vitamins can influence spermatogenesis. The aim of this project was to evaluate the effects of date palm gommule water/alcoholic extract on reproductive tissues, sex hormone and sperm quality of rat. Methods: Sev...

The aim of this study was to evaluate the chromatin status in different groups of patients. Five groups of men were selected: pre-vasectomy; male factor infertility; varicocele; immunological male infertility; and idiopathic infertility. Chromatin status was evaluated using flow cytometry after staining the DNA with the fluorochrome propidium iodide. Differences were observed in the state of sp...

Prior to ICSI the cumulus-oophorous-complex of the oocyte is commonly remove with impure animal-derived hyaluronidase of low strength. The lower strength of the enzyme, the high concentration of impurities and the necessity of extensive time-dependent mechanical denudation damages of the oocytes, increases the risk of lysing the oocytes and the transfer of animal-derived pathogens. All this can...

Background: Varicocele is related with poor semen quality and sperm chromatin integrity which possibility decreases the spontaneous pregnancy potential. Some of the varicocele individuals have normal semen parameters with failed pregnancy. It is also one of the most controversial issues in the field ofinfertility, especially regarding why, when and for whom varicocelectomy should be implemented...