Background CHO (Chinese Hamster Ovary) cells are the cell line of choice for therapeutic protein production. Although the achieved volumetric titers have increased significantly over the past two decades, the establishment of wellproducing CHO cell lines is still difficult and not always successful [1]. Factors influencing productivity are the chosen host cell line, the genetic vectors, applied...

This study examined the signal transduction correlates of mu opioid agonist efficacy in two systems: mu receptor-transfected mMOR-CHO cell and rat thalamic membranes. The potency and maximal stimulation of [35S]GTP gamma S binding by various agonists was measured in the presence of excess GDP and compared with receptor binding affinity under identical assay conditions. Results showed that the r...

Chinese hamster ovary (CHO) cells cultured in vitro were continuously exposed to increasing concentrations of diethylmaleate (DEM). Chronic exposure of these cells (designated CHO/DEM) to 80 microM diethylmaleate resulted in an increase in cystine transport, a decrease in glutathione inhibition of the enzyme gamma-glutamylcysteine synthetase, elevation of intracellular glutathione levels to 4.3...

Removal of S-Methylmethionine Sulfonium from Satsuma Mandarin Juice Yutaka OSAJIMA, Mitsuya SHIMODA, Hisao MAEDA* and Shozo UENO** Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka 812 * Wakayama Agricultural Processing Research Corporation , Momoyama-cho, Naga-gun, Wakayama-ken 649-61 ** Saga-ken Horticultural Growers Co-operative Association , Ogi-c...

Tricyclic antidepressants (TCAs) have been reported to interact with the opioid system, but their pharmacological activity at opioid receptors has not yet been elucidated. In the present study, we investigated the actions of amoxapine, amitriptyline, nortriptyline, desipramine, and imipramine at distinct cloned and native opioid receptors. In Chinese hamster ovary (CHO) cells expressing delta-o...

Carbohydrate (CHO) mouth rinse has been shown to improve time trial performance. Although the exact mechanism remains un-established, research postulates that there are oral cavity receptors which increase neural drive. Increasing the duration of the mouth rinse could potentially increase stimulation of these receptors. The aim of the current investigation was to determine whether the duration ...

Cyclophosphamide is used to treat a wide range of human malignancies. However, it is also a known carcinogen associated with induction of therapy-related leukemia and bladder cancer. The DNA repair protein, O-alkylguanine-DNA alkyltransferase (AGT), protects cells from the toxic and mutagenic effects of O-alkylating agents. We report here the contribution of AGT in protecting against the toxic ...

Background: Cholesterol oxidase (CHO) has various clinical and industrial applications. Recently, microbial CHO have received a great attention for their wide usage in medicine. Here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for CHO production and biochemical characterizations of produced CHO enzyme were described. Finally, CHO gene was cloned int...

background: cholesterol oxidase (cho) has various clinical and industrial applications. recently, microbial cho have received a great attention for their wide usage in medicine. here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for cho production and biochemical characterizations of produced cho enzyme were described. finally, cho gene was cloned int...

The agonist-bound gonadotropin-releasing hormone (GnRH) receptor engages several distinct signaling cascades, and it has recently been proposed that coupling of a single type of receptor to multiple G proteins (G(q), G(s), and G(i)) is responsible for this behavior. GnRH-dependent signaling was studied in gonadotropic alphaT3-1 cells endogenously expressing the murine receptor and in CHO-K1 (CH...