Although complex fractionated electrogram (CFE) is known to be a target for catheter ablation of fibrillation, its physiological meaning in fibrillation wave-dynamics remains to be clarified. We evaluated the spatiotemporal relationships among the parameters of fibrillation wave-dynamics by simulation modeling. We generated maps of CFE-cycle length (CFE-CL), local dominant frequency (LDF), wave...

IntroductIon Cerebral fat embolism (CFE) is a rare but potentially lethal disease that is usually complicated by long bone fracture, which can develop over 12–72 h. Since the main form of treatment for CFE is supportive, early diagnosis, prevention, and appropriate treatment can reduce mortality and the development of complications. However, due to symptom heterogeneity and imprecise diagnostic...

At many trichloroethene (TCE)-contaminated field sites, microbial transformation of TCE results in the accumulation of vinyl chloride (VC), a known carcinogen and neurotoxin. Quantitative tools are needed to determine the in situ rates of VC transformation to ethene in contaminated groundwater. For this study, E-/Z-chlorofluoroethene (E-/Z-CFE) was evaluated as a surrogate for VC in laboratory ...

There is evidence that the principles present in the widely consumed Indian food plant C. fimbriata extract (CFE) suppress appetite, and provide antiobesogenic and metabolic benefits. The Diet-Induced Obesity (DIO) rat model was used to investigate CFE's anorexigenic effects. Rats were randomly divided into three groups: (i) untreated control (C), (ii) control for cafeteria diet (CA), and (iii)...

Polycyclic aromatic hydrocarbons (PAHs) are a major class of organic hydrocarbons with high molecular weight that originate from both natural and anthropogenic sources. Sixteen PAHs are included in the U.S Environmental Protection agency list of priority pollutants due to their mutagenic, carcinogenic, toxic and teratogenic properties. In this study, the development and optimization of a simpli...

BACKGROUND Ablation of persistent atrial fibrillation can be challenging, often involving not only pulmonary vein isolation (PVI) but also additional linear lesions and ablation of complex fractionated electrograms (CFE). We examined the impact of stepwise ablation on a human model of advanced atrial substrate of persistent atrial fibrillation in heart failure. METHODS AND RESULTS In 30 patie...

INTRODUCTION The purity of genomic DNA (gDNA) extracted from different clinical specimens optimizes sensitivity of polymerase chain reaction (PCR) assays. This study attempted to compare two different DNA extraction techniques namely salting-out and classic phenol-chloroform. MATERIALS AND METHODS Qualification of two different DNA extraction techniques for 634 clinical specimens highly suspe...

Development of artificial cell models requires encapsulation of biomolecules within membrane-bound compartments. There have been limited studies of using mammalian cell-free expression (CFE) system as the 'cytosol' of artificial cells. We exploit glass capillary droplet microfluidics for the encapsulation of mammalian CFE within double emulsion templated vesicles. The complexity of the physicoc...

The gravimetric analysis of total fecal fat by a proposed chloroform extraction procedure has been shown to compare very closely with an accepted procedure involving a petroleum ether extraction, at all levelsof fecal fat. The decrease in the density of the chloroform by the fecal fat is used as a semiquantitative measure of fat concentration. This is accomplishedby noting the bouyancy of the c...

The petroleum ether (60-80o), chloroform methanol extracts of leaves and root bark of ficus glomerata (Moraceae) were prepared by successive solvent extraction followed by vacuum evaporation and the marc left after successive solvent extraction was extracts with boiling water to get aqueous extracts, all the extracts were subjected to qualitative chemical tests to find out phytoconstituents pre...