Renewed interest in chlamydia vaccination has revealed the need for a greater understanding of the seroprevalence of chlamydial infection in US populations. We used a Chlamydia trachomatis elementary body (EB)-based enzyme-linked immunosorbent assay to define the characteristics of the humoral immune response and to determine seroprevalence. Two groups were analyzed: one consisting of patients ...

The use of fluorescein-conjugated monoclonal antibody (Syva, UK) provided a rapid reliable diagnostic test for Chlamydia trachomatis in conjunctival samples from 100 adults with acute follicular conjunctivitis and seven babies with suspected chlamydial ophthalmia neonatorum. Elementary bodies (EBs) were seen in smears from 11 of the adults, and culture confirmed C. trachomatis infection in nine...

Western-blot analysis was used to study the reaction of koala antisera, two specific polyclonal antibodies and one monoclonal antibody, with chlamydial antigens in koalas infected with Chlamydia psittaci. The koala sera recognized four C. psittaci surface antigens, corresponding to the major outer membrane protein (39.5 kDa), 31 kDa protein, 18 kDa protein and lipopolysaccharide. The S25-23 LPS...

B-cell peptide epitopes in chlamydial heat shock protein 60 (hsp60) were elucidated with antisera from 13 rabbits immunized with Chlamydia trachomatis serovars B, C, and L2 and antisera from eight women with C. trachomatis-associated ectopic pregnancies. Thirteen major epitopes were identified with the human sera, 10 of which were also observed with rabbit antisera. Seven of the 13 epitopes rec...

Using a fluorescein labelled monoclonal antibody ("Micro Trak") to identify chlamydia elementary bodies in endocervical smears, we detected Chlamydia trachomatis in 31 (21%) of 150 cases of classic pelvic inflammatory diseases (PID) and in 42 (18%) of 232 cases of abdominal pain not diagnosed as PID. Only 43 (59%) of the women yielding chlamydiae would have received treatment in the absence of ...

The role that collectin (mannose-binding protein) may play in the host's defense against chlamydial infection was investigated. Recombinant human mannose-binding protein was used in the inhibition of cell culture infection by Chlamydia trachomatis (C/TW-3/OT, E/UW-5/Cx, and L2/434/Bu), Chlamydia pneumoniae (AR-39), and Chlamydia psittaci (6BC). Mannose-binding protein (MBP) inhibited infection ...

Clearview Chlamydia (Unipath) is a rapid monoclonal antibody based latex immunodiffusion test for detecting chlamydial antigen in endocervical specimens. The assay does not require specialised equipment or extensive training and takes less than 30 minutes from sample to results. The clinical performance of Clearview Chlamydia was evaluated with 478 paired endocervical swabs from patients attend...

Chlamydia trachomatis; infection; heat shock proteins hronic chlamydial infections have been documented in numerous works 1,z as silently evolving diseases. Evidence of resistance to antibiotic therapy, as demonstrated by positive cultures long after treatment in infertile women, 3,4 as well as positive chlamydial antigens or nucleic acids in culture-negative pelvic samples of infertile women s...

Chlamydia trachomatis; infection; heat shock proteins hronic chlamydial infections have been documented in numerous works 1,z as silently evolving diseases. Evidence of resistance to antibiotic therapy, as demonstrated by positive cultures long after treatment in infertile women, 3,4 as well as positive chlamydial antigens or nucleic acids in culture-negative pelvic samples of infertile women s...

The aim of this study was to evaluate the prevalence of serum immunoglobulin (Ig)G and IgA antibodies to recombinant chlamydial 60 kDa heat shock protein (C-hsp60) and to assess the prevalence of serum IgG antibodies to recombinant chlamydial 70 kDa heat shock protein (C-hsp70) in Chlamydia-associated chronic salpingitis and/or salpingitis isthmica nodosa with tubal occlusion. Infertile patient...