Objectives: To assess and compare the quantity quality of newly bone generated when using chitosan-based gel scaffold osteoprotegerin-chitosan scaffold. Methods: A total 18 critical-sized defects on New Zealand white rabbit craniums were created. In 12 defects, either chitosan or was implanted last six kept unfilled as a control. Bone formation examined at 6 weeks. Bone’s specimens scanned High...

Gelatin, chitosan and hyaluronic acid are natural components used to prepare polymeric scaffold in tissue engineering. The physical properties of these materials confer an appropriate microenvironment for cells, which can be used as a regeneration system for skin and cartilage. In this work, we prepared and characterized a Gelatin/Chitosan/Hyaluronan lyophilized-polymer. Physical properties of ...

We report a simple method to produce stable chitosan derivative nanofibers via electrospinning. A chitosan solution with lactate salt was electrospun to produce nanofibers, followed by thermal treatment to enhance fiber stability. Chemical and morphological analyses demonstrated that the resulting nanofibers were crosslinked via amidation between chitosan and lactate salt. These fibers exhibite...

Understanding the formation of biominerals like nacre can lead to the fabrication of more advanced biomimetic materials. Several factors are known to influence the final form of both native nacre and biomimetic synthetic variants. Two important components in calcium carbonate biominerals such as nacre are the organic scaffold and the acidic proteins. Interactions between these two components ma...

A biodegradable, biocompatible poly(chitosan-g-lactic acid) (PCLA) scaffold was prepared and evaluated in vitro and in vivo. The PCLA scaffold was obtained by grafting lactic acid (LA) onto the amino groups on chitosan (CS) without a catalyst. The PCLA scaffolds were characterized by Fourier Transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The biodegradabilty was ...

Biomaterial surfaces and their nanostructures can significantly influence cell growth and viability. Thus, manipulating surface characteristics of scaffolds can be a potential strategy to control cell functions for stem cell tissue engineering. In this study, in order to construct a hydroxyapatite (HAp) coated genipin-chitosan conjugation scaffold (HGCCS) with a well-defined HAp nanostructured ...

The advent of biocompatible and biodegradable scaffolds has opened up a plethora possibilities in neural tissue engineering. An emerging approach to overcome some existing drawbacks scaffold design functioning is natural conductive polymeric scaffold. It analogous the extracellular matrix provides an environment conducive growth. This study focuses on fabrication soft scaffold, reinforced with ...

PURPOSE The aim of this prospective study was to evaluate whether blending two kinds of biomaterials, chitosan and polycaprolactone (PCL), can be used as scaffold and carrier for growth and differentiation of corneal endothelial cells (CECs). METHODS A transparent, biocompatible carrier with cultured CECs on scaffold would be a perfect replacement graft. In the initial part of experiment, for...

An effective paradigm for transplanting large numbers of neural stem cells after central nervous system (CNS) injury has yet to be established. Biomaterial scaffolds have shown promise in cell transplantation and in regenerative medicine, but improved scaffolds are needed. In this study we designed and optimized multifunctional and biocompatible chitosan-based films and microspheres for the del...